52例稽留流产绒毛染色体高通量基因测序  

Villi Chromosome High- throughput Gene Sequencing of 52 Cases of Missed Abortion

作　　者：陈悦[1] 马钰[1] 王翠荣[1] 

机构地区：[1]广州医科大学附属第五医院,广东广州510700

出　　处：《现代医院》2016年第1期17-18,21,共3页Modern Hospitals

基　　金：2014年广州市黄埔区科技和信息化局应用基础研究计划项目(编号:201444-15)

摘　　要：目的通过高通量基因测序检查稽留流产胚胎绒毛组织染色体,了解胚胎染色体异常与稽留流产之间的关系,为指导下一次妊娠提供依据。方法用高通量基因测序技术,对52例稽留流产绒毛组织物进行染色体检测,了解染色体异常的类型及所占比例。结果 52例稽留流产绒毛样本中,染色体异常28例,数目异常24例,其中三体17例,三倍体6例,单体1例;结构异常4例。结论胚胎染色体异常是早孕期稽留流产的主要原因,高通量基因测序技术检测绒毛染色体具有全面、快速、准确的优点,有助于明确稽留流产病因,合理指导再次妊娠。Objective To understand the relationship between embryonic chromosomal abnormalities and missed abortion by high - throughput gene sequencing, so as to provide a basis for guiding the next pregnancy. Methoils By chromosome detection on villi chromosome of 52 cases of missed abortion by high - throughput gene sequencing technique, the types of chromosome abnormalities and the proportion were investigated. Results Among villi samples from the 52 cases of missed abortion, chromosomal abnormalities were founded in 28 cases, including 24 cases of number abnormality （ 17 cases of trisomy, 6 cases of triploid and a case of monomer） and 4 cases of structural abnormalities. Conclusion Embryonic chromosomal abnormality is the main cause of missed abortion in early pregnancy. High - throughput gene sequencing technology used to detect the villi chromosome is comprehensive, fast and accurate, helping to clarify the cause of missed abortion and guide the next pregnancy reasonably.

关 键 词：高通量基因测序技术 稽留流产 绒毛 染色体核型分析 

分 类 号：R714.21[医药卫生—妇产科学] R394[医药卫生—临床医学]