不同剂量血必净对大鼠心肺复苏后早期血清NSE及S-10013蛋白的影响  

Effects by different dose of Xuebijing Injection on early time NSE and S - 100 beta protein after cardiopulmonary resuscitation in rats

作　　者：刘海健[1] 吕少君[1] 徐志勇[1] 黄丁丁[1] 刘荣[1] 翁浩[1] 

机构地区：[1]上海交通大学附属第六人民医院南院(上海市奉贤区中心医院)麻醉科,上海201499

出　　处：《中国急救医学》2016年第1期83-85,共3页Chinese Journal of Critical Care Medicine

基　　金：基金项目：上海市卫生局青年科研项目（20114Y138）

摘　　要：目的观察不同剂量血必净对大鼠心肺复苏后早期血清神经元特异性烯醇化酶（NSE）及S-10013蛋白的影响。方法取12周龄SD大鼠32只，体质量（300±20）g，随机分为四组，每组8只。A组（安慰剂组）：进行气管切开插管、右股动脉置管测压，待自主循环恢复（ROSC）后给予生理盐水8mL／kg；B组（低剂量组）：自主循环恢复后给予生理盐水4mlMkg＋血必净4mlMkg；C组（中剂量组）：自主循环恢复后给予生理盐水2mL／kg＋血必净6mL／kg；D组（高剂量组）：自主循环恢复后给予血必净8mlMkg。记录每只大鼠窒息致CA时间、CPR开始至ROSC时间、ROSC率、24h内所使用生理盐水和多巴胺的总量。测量自主循环恢复后24h血清NSE及S-100β蛋白浓度。结果与A组比较，B、C、D组多巴胺（mg）（3526±141vs．1865±189、1376±197、1215±14．5）、生理盐水用量（mL）（5．5±1．1vs．4．2±1．2、2．6±1．0、2．5±1．0），NSE（mg／L）（8771±91vs．6957±812、5712±836、5324±665）及S-100β（μg/L）（443±67VS．312±61、231±59、2114-62）蛋白均减少（P〈0．05）；与B组比较，C、D组多巴胺用量、生理盐水用量、NSE及S-100β蛋白均减少（P〈0．05）；C、D组间比较差异无统计学意义（P〉0．05）。结论血必净6mL／kg即可显示出良好的脑保护作用。Objective To observe the early effects by different dose of Xuebijing Injection on NSE and S - 100 beta protein after cardiopulmonary resuscitation（CPR） in rats. Methods 32 healthy SD rats aged 12 weeks weighting （300 ± 20） g were randomLy divided into 4 groups（n = 8 each）. Group A （group operation -control） ： only intubated through the tracheal and the right femoral artery, saline 8 mL/kg were given after ROSC. Group B （ group low dose ） ： saline 4 mL/kg ＋ xuebijing 4 mL/kg were given after ROSC. Group C（ group middle dose ） ： saline 2 mL/kg ＋ xuebijing 6 mL/kg were given after ROSC. Group D （group high dose ） ： xuebijing 8 mL/kg were given after ROSC. Time of CA and ROSC, ROSC proportion and the amount of saline and dopamine in 24 h were recorded . NSE and S - 100 beta protein were also measured 24 h later after ROSC. Results Compared with group A, the amount of saline and dopamine in 24 h, NSE and S - 100 beta protein were all lower in group B, C and D （P 〈 0.05） ; Compared with group B, the amount of saline and dopamine in 24 h, NSE and S - 100 beta protein were all lower in groupC and D （P 〈 0.05 ） ; but there were not significant difference in group C and D（P 〉 0.05 ）. Conclusion Xuebijing 6 mL/kg showed the excellent effect for the brain protection.

关 键 词：血必净 心肺复苏(CPR) 脑保护 神经元特异性烯醇化酶(NSE) 

分 类 号：R473.5[医药卫生—护理学]