褪黑激素对休情期银黑狐腔前卵泡卵母细胞超微结构的影响  

The impact of melatonin on the ultrastructure of anestrus Vulpes fulva preantral follicle oocytes

作　　者：司志文 赵英[1] 马泽芳[1] 崔凯[1] 史雪萍[1] 

机构地区：[1]青岛农业大学动物科技学院,青岛266109

出　　处：《兽类学报》2016年第1期112-117,共6页Acta Theriologica Sinica

基　　金：山东省毛皮动物产业技术体系创新团队项目(SDAIT-18-011-01);国家科技部公益性行业(农业)科研专项;山东省农业重大应用技术创新项目(鲁财农指[2015]16)

摘　　要：为了研究褪黑激素(Melatonin,MLT)对休情期银黑狐腔前卵泡卵母细胞超微结构的影响。本研究选取健康7月龄埋植和未埋植MLT的银黑狐各5只,取其左侧卵巢共计10枚,制备超薄切片后利用透射电镜分别观察每枚卵巢的各级腔前卵泡各1-5个,并进行拍照。结果埋植和未埋植MLT的银黑狐原始卵泡卵母细胞内均有少量线粒体和高尔基体,而未埋植MLT的银黑狐卵母细胞中还可见少量滑面内质网;初级卵泡卵母细胞内,均开始形成不完整透明带,线粒体及内质网数量均有所增加,沿透明带出现少量皮质颗粒;次级卵泡阶段,未埋植MLT银黑狐卵母细胞微绒毛数量较埋植MLT的多,其余细胞器未见差异。结果表明,MLT对休情期银黑狐卵巢腔前卵泡卵母细胞的线粒体、脂滴、高尔基体、皮质颗粒等细胞器的发育没有影响,仅对初级卵泡阶段内质网的发育有抑制作用。This study aimed to explore the impact of melatonin（ MLT） on the ultrastructure of oocytes in preantral follicle of Vulpes fulva during the anestrus stage. Five left side ovaries for each group were collected from seven month old healthy MLT implanted or non-MLT implanted Vulpes fulva,respectively. The ultrastructures of 1- 5 follicles from different stages in each ovary were examined by transmission electron microscope. A few mitochondria and golgi apparatus in primordial follicles were detected in both MLT implanted and non-MLT implanted Vulpes fulva. A small amount of smooth endoplasmic reticulum was found only in the primordial follicles of non- MLT implanted Vulpes fulva. Zona pellucida started to form in primary follicles with a few of coritical granules along the zona pellucida. And the number of mitochondria and endoplasmic reticulum increased in primary follicles in both groups. The number of zona microvilli in secondary follicle oocytes of nonMLT implanted Vulpes fulva was greater than that of MLT implanted Vulpes fulva. The results suggest that MLT only impacts the development of endoplasmic reticulum in Vulpes fulva primary follicles at anestrus stage.

关 键 词：褪黑激素 银黑狐 休情期 腔前卵泡 卵母细胞 超微结构 

分 类 号：S865.23[农业科学—野生动物驯养]