1,25-二羟维生素D_3对IgA肾病大鼠肾组织中白细胞介素-4表达的影响  

Effect of 1,25-dihydroxyvitamin D_3 on expression of IL-4 in renal tissues of rats with IgA nephropathy

作　　者：马勇[1] 罗静[2] 郭珲[1] 

机构地区：[1]山西医科大学第二医院肾内科,太原030001 [2]山西医科大学第二医院风湿科,太原030001

出　　处：《中国药物与临床》2016年第1期12-15,共4页Chinese Remedies & Clinics

基　　金：山西省科技攻关项目(20130313022-3);山西省回国留学人员科研资助项目(晋留管办发〔2014〕9号);山西省出国留学基金(2013117);山西省国际合作项目(2013081056);太原市科技资助项目(12016904)

摘　　要：目的研究1,25-二羟维生素D_3[1,25(OH)_2D_3]对Ig A肾病(Ig AN)大鼠肾组织中白细胞介素-4(IL-4)的影响。方法将造模成功的32只Ig AN大鼠模型随机分为未干预组、1,25(OH)_2D_3治疗组、泼尼松组、1,25(OH)_2D_3+泼尼松组,同时设立正常对照组。应用免疫组织化学的方法检测大鼠肾脏组织中IL-4表达的变化。收集Ig AN大鼠的尿蛋白定量(24 h)及尿红细胞计数等生化指标,分别对各组IL-4表达情况与相应的生化指标进行相关性分析。结果 1尿蛋白定量(24 h):正常对照组(A组)为(6.9±0.6)mg/24 h;未干预组(B组)为(50.8±2.9)mg/24 h;泼尼松组(C组)为(12.1±1.3)mg/24 h;1,25(OH)_2D_3治疗组(D组)为(25.4±1.0)mg/24 h;泼尼松+1,25(OH)_2D_3治疗组(E组)为(9.6±0.6)mg/24 h;经治疗后C、D、E组尿蛋白定量(24 h)较B组均有下降(P<0.05),其中E组下降最显著(P<0.05);2尿红细胞计数:A组为(3.3±1.5)个/μl;B组为(377.4±11.1)个/μl;C组为(19.3±2.7)个/μl;D组为(196.6±7.3)个/μl;E组为(9.3±2.2)个/μl;经治疗后C、D、E组尿红细胞计数较B组均有下降(P<0.05),其中E组下降最显著(P<0.05);3IL-4平均光密度值:A组为0.080±0.030,B组为0.230±0.013,C组为0.162±0.021,D组为0.199±0.031,E组为0.092±0.024;经治疗后C、D、E组平均光密度较B组均有下降(P<0.05),其中E组下降最显著(P<0.05);4IL-4的表达与尿蛋白定量(24 h)呈正相关(P<0.05),与尿红细胞计数无明显相关性。结论 1,25(OH)_2D_3下调了肾组织内的IL-4的表达,调节Ig AN大鼠的自身免疫性炎症反应。Objective To investigate the effect of 1,25-dihydroxyvitamin D3 [1,25（OH）2D3] on the expression of interleukin-4 （IL-4） in renal tissues of rats with IgA nephropathy（IgAN）. Methods After successful modeling, 32 I- gAN rat models were randomly divided into the nonintervention group, 1,25（OH）2D3 treatment group, prednisone group, 1,25（OH）2D3＋prednisonc group, and normal control group. Immunohistochemistry was used to determine the changes of the IL-4 expression in renal tissue of rats. The biochemical indicators of 24-hour urinary protein and red blood cell count in IgAN rats were collected. The IL-4 expression and the corresponding biochemical indicators in each group were performed for correlation analysis in each group, respectively. Results ①The 24-hour quantitative urinary pro-tein in the normal control group （group A）, nonintervention group （group B）, prednisone group （group C）, 1,25（OH）2D3 treatment group （group D） and 1,25（OH）2D3＋prednisone group （group E） was （6.9±0.6）mg/24 h, （50.8±2.9） mg/24 h, （12.1±1.3）mg/24 h, （25.4±1.0）mg/24 h, （9.6±0.6）mg/24 h, respectively. After treatment, the urinary protein in group C, D and E were decreased compared with that in group B （P〈0.05）, and group E decreased most significantly （P〈0.05）. ②The urinary red blood cell count in group A, B, C, D and E was （3.3±1.5）/μ1, （377.4±11.1）/μl, （19.3±2.7）/μl, （196.6±7.3）/μl, and （9.3±2.2）/μl, respectively. After treatment, the urinary red blood cell count in group C, D and E were decreased compared with that in group B （P〈0.05）, and group E decreased most significantly （P〈0.05）. ③The IL- 4 mean optical density in group A, B, C, D and E was 0.080±0.030, 0.230±0.013, 0.162±0.021, 0.199±0.031, and 0.092±0.024, respectively. After treatment, the mean optical density in group C, D and E were decreased compared with that in group B （P〈0.05）, and group E decreased most significantly （P〈0.05

关 键 词：肾小球肾炎 IGA 骨化三醇 白细胞介素 

分 类 号：R692.31[医药卫生—泌尿科学]