昆虫细胞无血清培养基研究进展  

Progress on Insect Cell Serum-free Media

作　　者：马伟 王家敏 令世鑫 马桂兰 马花 乔自林 马忠仁 冯玉萍 

机构地区：[1]甘肃省动物细胞工程技术研究中心,甘肃兰州730030 [2]兰州民海生物工程有限公司,甘肃兰州730010 [3]生物工程与技术国家民委重点实验室,甘肃兰州730030

出　　处：《动物医学进展》2016年第2期101-104,共4页Progress In Veterinary Medicine

基　　金：教育部"长江学者和创新团队发展计划"项目(IRT13091);西北民族大学中央高校基本科研业务费专项资金项目(31920150029);兰州市科技计划项目(2013-4-101);甘肃省农业生物技术专项(GNSW-2014-24)

摘　　要：随着昆虫杆状病毒表达载体系统的广泛应用,昆虫细胞大规模无血清培养已成为一种发展趋势。目前的昆虫细胞培养基中主要有糖类、维生素、氨基酸、脂类、无机盐、有机酸等基础成分,此外还需要添加血清或酵母提取物和水解乳蛋白等血清替代物。然而使用血清会带来诸多难以解决的问题,因此开发使用血清替代物和无血清培养基已成为生物制品行业关注的主要方向。论文综述了昆虫细胞无血清培养基的研究进展,包括研究历程、研究现状、基础成分和其他添加物等。With the wide application of insect baculovirus expression vector system, the large scale of insect cell culture has become a developing trend. At present, there are some basic elements such as sugars, vita- mins, amino acids, lipids, inorganic salts and organic acids in the insect cell culture media, and it also needs to add serum or serum substitutes like yeastolate and lactalbumin hydrolysate. However, serum will bring many difficulties if being used, so developing and using the serum substitutes and serum-free media has become the main research direction of the biological product industry. This paper summarized the re- search progress on insect cell serum-free media, including the research history, research status, basic com- ponents and other additives, etc..

关 键 词：昆虫细胞 无血清培养基 血清替代物 

分 类 号：Q813.11[生物学—生物工程]