白及止血海绵止血作用的实验研究  

Study on hemostasis of Bletillastriata hemostatic sponge

作　　者：王巍[1] 程明和[2] 高静[2] 张晓洁 

机构地区：[1]解放军455医院,上海200052 [2]第二军医大学药学院,上海200433 [3]总后丰台综合仓库药材供应站,北京100071

出　　处：《药学实践杂志》2016年第1期32-35,65,共5页Journal of Pharmaceutical Practice

基　　金：南京军区"十一五"医药卫生科研基金(12MA020)

摘　　要：目的探讨白及止血海绵的止血作用。方法制备大鼠断尾出血模型,采用家兔建立肝脏、脾脏创伤出血模型以及Beagle犬肝脏、腹主动脉创伤出血模型,分别用空白对照、吸收性明胶海绵和白及止血海绵进行止血,通过观察止血时间、出血量、组织病理学变化等指标,评价白及止血海绵的止血效果和体内吸收情况。结果大鼠断尾止血实验中,白及止血海绵组在夹闭1min后可以有效止血。与空白对照组比较,白及止血海绵对大鼠断尾出血模型有显著的止血作用(P<0.01)。在家兔肝脏、脾脏创伤止血实验中,与吸收性明胶海绵组比较,白及止血海绵能明显缩短家兔肝脏、脾脏创伤出血时间(P<0.05),并明显减少脾脏出血量(P<0.05)和肝脏出血量(P<0.01)。与吸收性明胶海绵组比较,白及止血海绵组Beagle犬肝脏和腹主动脉创伤出血量明显减少(P<0.05),对Beagle犬肝脏和腹主动脉创伤出血有显著止血作用。体内降解实验中观察到白及止血海绵可在体内降解吸收。组织病理学观察显示,经白及止血海绵止血后的肝脏、脾脏创面组织周围未见明显病理改变。结论白及止血海绵具有较好的止血作用,止血效果明显优于吸收性明胶海绵,是一种较好的可吸收性止血材料。Objective To investigate the hemostatic effect of Bletillastriata hemostatic sponge. Methods Tail hemor- rhage model in rats was preparation. Another model was established in liver and spleen hemorrhagic model of rabbits, Beagle dog＇s abdominal aorta and liver were made. Hemostasis was performed with medical gauze, gelatin sponge and Bletillastriata hemostatic sponge. The hemostatic effects were evaluated by total blood loss, hemostatic time and histological observation. Observation of Bletillastriata hemostatic sponge change was completed to determine their degradation in the bodies. Results Rats tail hemorrhage was clamped, after 1 rain, Bletillastriata hemostatic sponge could effectively stop the bleeding. The Bletillastriata hemostatic sponge has good hemostatic effect of rats tail hemorrhage model compared to blank group（P〈0.01）. Compare with the gelatin sponge group, the Bletillastriata hemostatic sponge could significantly shorten the liver and spleen bleeding time（P〈0.05） , decrease the volume of the spleen bleeding（P〈0.05） and the liver bleeding（P〈0.01）, in hemor- rhagic model of rabbits. Compared with the gelatin sponge group, Bletillastriata hemostatic sponge could more effectively stop the bleeding in the abdominal aorta and liver model of Beagle dogs （P〈0.05）. The Bletillastriata hemostatic sponge could be degraded in vivo. Histologic study revealed the Bletillastriata hemostatic sponge was no significant pathological change around the liver and spleen. Conclusion The BgetiHastriata hemostatic sponge has good hemostatic effect. The hemostatic effect of Bletillastriata hemostatic sponge is better than gelatin sponge, which could be a good topical hemostatic material.

关 键 词：白及 止血 明胶海绵 创伤 

分 类 号：R965[医药卫生—药理学]