高效液相色谱法测定人血浆中阿克他利的浓度及其药动学研究  

Study on pharmacokinetics and determination of actarit in human plasma by HPLC

作　　者：何娟[1] 周成杰 

机构地区：[1]上海中医药大学附属曙光医院药剂科,上海201203 [2]上海新药研究中心,上海201203

出　　处：《药学实践杂志》2016年第1期41-43,75,共4页Journal of Pharmaceutical Practice

摘　　要：目的 建立人血浆中阿克他利浓度的高效液相色谱检测方法。方法 血浆样品采用液-液萃取的方法,采用Dikma C18柱分离,以水（含1.2%乙酸）-甲醇（50∶50,V/V）的混合溶液为流动相,检测波长为245nm,流速为1.0ml/min,柱温30℃。结果 建立的人血浆内阿克他利测定方法线性范围为75~4 000ng/ml,定量下限可达75ng/ml。日内、日间精密度（RSD）均小于10%,方法准确度大于90%,提取回收率大于88%。结论 该方法快速、灵敏、专属,适用于人血浆中阿克他利浓度的测定。Objective To develop a simple and sensitive HPLC-UV method to determined actarit in plasma. Methods The chromatographic separation was carried out on a Dikma C18 column with a mobile phase composed of water containing 1.2% acetic acid-methanol （50 ： 50, V/V） at a flow rate of 1.0 ml/min and the temperature of column was 30℃. UV wave- length mode was monitored at 245 nm. Results A linear response function was established for the range of concentrations 75- 4 000 ng/ml for actarit. The inter- and intra-day precision value were less than 10% for actarit and the accuracy was more than 90%. The extraction recovery was more than 88%. Conclusion This simple, sensitive, and exclusive method could be used in routine clinical practice to monitor actarit concentrations in human plasma.

关 键 词：阿克他利 高效液相色谱法 药动学 

分 类 号：R927[医药卫生—药学] R945