云南省昆明地区种鸡J亚群禽白血病血清学调查  

Serological Survey on J Subgroup of Avian Leukemia of Breeding Chicken in Kunming Area of Yunnan Province

作　　者：白马伟 赵谦[2] 和贵斌 张正学[2] 

机构地区：[1]云南省蒙自市动物卫生监督所,云南蒙自661199 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《安徽农业科学》2016年第2期88-89,118,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]了解云南省昆明地区种鸡场J亚群禽白血病的感染状况。[方法]从云南省昆明市疑似感染禽白血病的某种鸡场采集644份泄殖腔(公鸡)拭子和460份蛋清(母鸡)样品,采用ELISA法对种鸡J亚群禽白血病进行抗原检测。[结果]在检测的460份蛋清样品中,172份抗原呈阳性,阳性率为37.39%;在检测的644份泄殖腔拭子中,520份抗原呈阳性,阳性率为80.75%。A养殖场共检测644份样品,总阳性率为63.66%,其中蛋清样品的阳性率为39.13%,泄殖腔样品的阳性率为82.07%。B养殖场共检测460份样品,总阳性率为61.30%,其中蛋清样品的阳性率为34.78%,泄殖腔样品的阳性率为78.99%。此次检测结果表明云南省昆明地区种鸡群已严重感染J亚群禽白血病。[结论]该研究可为种鸡J亚群禽白血病的防治提供理论依据。[ Objective ] To understand the infection situation of J subgroup of avian leukemia （ALV） in breeding chicken farm in Kunming City of Yunnan Province. [ Method] 644 cloaca swabs of cock and 460 albumen samples of hens were collected from a breeding chicken farm infected with suspected avian leukemia virus in Kunming City of Yunnan Province. The antigen of J subgroup of avian leukemia in breeding chicken was detected by using ELISA. [ Result] Among 460 detected albumen samples, 172 antigen samples were positive, the positive rate was 37.39%. Among 644 cloaca swabs, 520 antigen samples were positive, the positive rate was 80.75%. 644 samples were detected in farm A, and the total positive rate was 63.66%. Among the detected samples, the positive rate of albumen samples was 39.13%, and the positive rate of cloaca swabs was 82.07%. 460 samples were detected in farm B, the total positive rate was 61.30%. Among them, the positive rate of albumen samples was 34.78% , and the positive rate of cloaca swabs was 78.99%. The detection results showed that the breeding chicken in Kunming City of Yunnan Province had been severely infected with J subgroup of ALV. [ Conclusion ] The research can provide theoretical basis for the prevention and control of J subgroup of ALV of breeding chicken.

关 键 词：禽白血病 J亚群 P27蛋白 ELISA 

分 类 号：S852.5[农业科学—基础兽医学]