大孔吸附树脂纯化常春藤皂苷C工艺研究  

Purifying process of hederacoside C by macroreticular resin

作　　者：曾建刚 孙化鹏[2] 阮琴妹 张珉[3] 钟晓红[2] 

机构地区：[1]汉寿县林业局,湖南汉寿415900 [2]湖南农业大学,湖南长沙410128 [3]湖南省林业科学院,湖南长沙410004

出　　处：《湖南林业科技》2016年第1期53-60,共8页Hunan Forestry Science & Technology

基　　金：湖南省教育厅科研项目(15A089);湖南省研究生科研创新项目(CX2014B290)

摘　　要：通过静态吸附试验比较7种树脂对常春藤皂苷C的吸附与解吸,筛选出效果最佳树脂,通过动态吸附试验对最佳树脂的上样p H、上样体积、洗脱液浓度、洗脱体积、洗脱流速进行优化。结果表明:HPD-100树脂对常春藤皂苷C的吸附与解吸性能最好,HPD-100树脂对常春藤皂苷C纯化的最佳条件为:上样体积为6BV,洗脱液乙醇浓度为80%,洗脱体积为7 BV,洗脱流速为1 BV/h。The best macroreticular resin was selected by comparing adsorption and elution performance of seven kinds of macroreticular resin through static adsorption experiment. And purifying process parameters such as the pH and volume of sample loading, concentration of washing solution, elution volume, and elution flow rate were optimized through dynam- ic state absorbance experiment. The results showed that HPD- 100 macroreticular resin had the best adsorption and elution performance. The optimum conditions of purification of hederacoside C were sample loading volume on 6 BV, elution vol- ume on 7 BV with 80% ethanol, elntion velocity was 1 BV/h.

关 键 词：常春藤皂苷C 吸附 洗脱 大孔吸附树脂 

分 类 号：TQ464.3[化学工程—制药化工]