萘酚电化学传感器的构建及其应用研究  

Construction and application of electrochemical sensors for the detection of naphthol

作　　者：任聚杰[1] 李柳佳 崔敏[1] 翟淼[1] 于聪聪[1] 籍雪平[2] 

机构地区：[1]河北科技大学理学院,河北石家庄050018 [2]河北医科大学基础医学院,河北石家庄050017

出　　处：《河北科技大学学报》2016年第1期39-46,共8页Journal of Hebei University of Science and Technology

基　　金：河北省教育厅科研项目(ZH2012078)

摘　　要：构建了一种基于聚L-半胱氨酸/氧化石墨烯复合材料修饰玻碳电极的萘酚电化学传感器,并用于1-萘酚（1-NAP）和2-萘酚（2-NAP）两种同分异构体的同时检测。采用循环伏安法（CV）及差分脉冲伏安法（DPV）考察了1-NAP和2-NAP在该复合膜修饰电极上的电化学行为,并对电极的修饰及萘酚同分异构体的检测条件进行了优化。结果显示,在pH值为7.5的0.1mol/L PBS缓冲液中,复合膜修饰电极对萘酚的电化学氧化具有较强的催化活性,两者的峰电位差约为0.182V,能基本达到萘酚同分异构体的氧化峰分离。在优化的实验条件下,采用差分脉冲伏安法（DPV）进行测定,发现1-NAP和2-NAP分别在2~40μmol/L和1~40μmol/L的浓度范围内,与其对应氧化峰电流呈良好线性关系,其中1-NAP检出限为0.19μmol/L（S/N=3）,2-NAP检出限为0.12μmol/L（S/N=3）。另外,此修饰电极在检测中还表现出良好的稳定性和重现性,具有较强的抗干扰能力。将修饰电极用于实际水样品中1-NAP和2-NAP的测定,其加标回收率分别为98.9%~101.7%和97.7%~102.1%。A poly（L-cysteine）/graphene oxide composite modified glassy carbon naphthol electrode is constructed,which is used for simultaneous determination of 1-naphthol（1-NAP）and 2-naphthol（2-NAP）.The electrochemical behavior of 1-NAP and 2-NAP on the modified electrode are investigated by cyclic voltammetry（CV）and differential pulse voltammetry（DPV）,and the conditions to modify the electrode and to detect naphthol isomers are optimized.The results show that the modified electrode has an excellent electrocatalytic activity in the oxidation of the naphthol in phosphate buffered solution of 0.1mol/L PBS（pH 7.5）.The difference of oxidation peak potential between 1-naphthol and 2-naphthol could reach 0.182 V,which is almost large enough to achieve the simultaneous detection for 1-naphthol and 2-naphthol in a mixture solution.Under optimizedexperimental conditions,differential pulse voltammetry is adopted to detect naphthol isomer.It is found that the oxidation peak currents of 1-NAP and 2-NAP show a good linear relationship with the concentration in the range of 2~40μmol/L and 1~40μmol/L,respectively.The detection limits（S/N=3）for 1-NAP and 2-NAP are 0.19μmol/L and 0.12μmol/L,respectively.The modified electrode shows good stability and reproducibility and has strong anti-interference ability in the detection.The electrode is applied to determine 1-NAP and 2-NAP in real water samples,and the average recoveries are in the range of 98.9%to101.7% and 97.7%to 102.1%,respectively.

关 键 词：电化学分析 萘酚 电化学传感器 聚L-半胱氨酸 氧化石墨烯 

分 类 号：O657.1[理学—分析化学]