柴达木盆地梭梭P5CS基因的扩增及序列分析  

Amplication and Sequence Analysis of P5CS Gene in Haloxylon ammodendronin Qaidam Basin

作　　者：马玉花[1] 冶贵生[1] 马娟[1] 冶桂莲 龙晓晨 李玉英[1] 马秀芳[1] 

机构地区：[1]青海大学农牧学院,青海西宁810016

出　　处：《西北林学院学报》2016年第1期88-91,共4页Journal of Northwest Forestry University

基　　金：青海省科技厅自然科学基金项目(2012-Z-938Q)

摘　　要：以柴达木盆地梭梭同化枝为材料,扩增其P5CS基因并进行序列测定。结果表明:所得柴达木盆地梭梭P5CS基因的碱基数量为442个,编码146个氨基酸。另外,同源性比对分析结果显示梭梭与甜菜、可可等植物的P5CS基因具有较高的同源性。研究结果将为下一步梭梭P5CS基因的结构与功能研究奠定基础。The fragment of P5CS gene of assimilating branches of Haloxylon ammodendron in Qaidam Basin was amplified and sequenced by the specific primers,then the nucleotide sequence and amino acid se- quence of P5CS gene was identified and summarized. The sequence analysis indicated that the total length of P5CS geng was 442 bp to encode 146AA. Homology analysis showed that there had high homology among Haloxylon ammodendron P SCS gene and other plants. This results would lay foundation for the further study of the structure of PSCS gene and it＇s function in H. ammodendron.

关 键 词：梭梭 P5CS基因 扩增 序列分析 

分 类 号：S727.23[农业科学—林木遗传育种]