东亚唐棣增殖培养条件的优化  

Optimization on Shoot Proliferation Culture Media of Amelanchier asiatica

作　　者：何志宇[1] 尹鹏先 程林林[1] 蔺小丽[1] 蔡靖[1] 姜在民[2] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]西北农林科技大学生命科学学院,陕西杨陵712100

出　　处：《西北林学院学报》2016年第1期126-129,共4页Journal of Northwest Forestry University

基　　金：林业公益性行业科研专项(201204308)

摘　　要：以东亚唐棣(Amelanchier asiatica)无菌试管苗为材料,研究了不同基本培养基、激素组合、蔗糖浓度以及琼脂浓度对东亚唐棣增殖的影响。结果表明:最为适合东亚唐棣增殖的培养基组合为MS+6-BA 1.00mg·L^(-1)+NAA 0.05mg·L^(-1)+IBA 0.10mg·L^(-1)+40g·L^(-1)蔗糖+8g·L^(-1)琼脂,增殖系数可达6.83,幼苗叶片生长舒展,苗高和茎的生长量也有了很大提高。试验结果将为东亚唐棣工厂化育苗提供技术指导。The objective of this study was to examine the influences of different basic media, hormone combi- nation,sucrose concentration, agar concentration on shoot proliferation of Amelanchier asiatica. The re- sults showed that an optimum medium was MS supplemented with 1.00 mg · L^-1 6-BA ,0.05 mg · L^-1 NAA,0.10 mg · L^-1 IBA ,40 g · L^-1 sucrose and 8 g ·L ~ agar,by which the multiplication coefficient reached 6.83. The height and quality of the shoots were greatly improved. This system could be used for rapid mass clonal propagation of A. asiatica for commercial production.

关 键 词：东亚唐棣 组织培养 优化 增殖 

分 类 号：S503.53[农业科学—作物学]