改良后细菌纤维素人工小口径血管的体外实验研究  

Hemocompatibility Evaluation in Vitro of Small-Caliber Modified Bacterial Cellulose

作　　者：黄涛[1] 王甩艳 赖琛[2] 奚廷斐[1,2] 

机构地区：[1]温州医科大学检验医学院生命科学学院,温州325035 [2]北京大学深圳研究院人体组织再生与修复深圳重点实验室,深圳518057

出　　处：《中国医药导刊》2015年第B05期9-13,共5页Chinese Journal of Medicinal Guide

基　　金：国家科技支撑计划(2012BAI18B06);课题名称:支架;关节;人造血管等高端植介入重大产品研发-小口径人工血管

摘　　要：目的:研究氧化细菌纤维素/蚕丝蛋白复合小口径人工血管的血液相容性及细胞毒性,探讨复合材料做人工血管材料的可行性。方法:采用TEMPO、NaClO_2和NaClO分别作为催化剂、氧化剂、和助催化剂,将细菌纤维素C6位上的羟基选择性氧化为羧基。再将氧化的细菌纤维素与10%(wt%)蚕丝蛋白溶液相混合,制成复合材料。体外溶血溶血试验来检测复合材料的溶血安全问题,并且进行血小板黏附实验和蛋白吸附实验来评价复合血管的血液相容性。通过MTT细胞毒性实验评价材料的细胞相容性。结果:血小板黏附、溶血实验和蛋白质吸附结果是低血小板黏附和蛋白吸附,且不引起溶血。细胞毒性实验表明复合物生物相容性良好。结论:氧化后细菌纤维素与蚕丝蛋白复合物在小口径人工血管的应用方面有一定的前景。Objective:To evaluate the hemocompatibility and cell cytotoxic effect of oxidized bacterial cellulose/silk fibroin composite product.Method:Using TEMPO,NaC10_2 and NaClO respectively as a catalyst,oxidant and cocatalyst,the C6 primary hydroxyls of the bacterial cellulose were selectively oxidized to form carboxylate groups.The composite products were generated by mixing of the oxidized bacterial cellulose with10%(wt%) silk fibroin.Hemolytic safety problems of composite materials were evaluated by hemolytic test in vitro to detect,and the blood compatibility of composite materials was evaluated by platelet adhesion and protein adsorption.The cell compatibility of materials was evaluated by MTT cell toxicity experiments.Result:The platelet adhesion,hemolytic test and protein adsorption results exhibited that the sample had significantly excellent blood compatibility featured by lower platelet adhesion and protein adsorption without causing hemolysis.Cytotoxicity experiments indicated that the sample had good cell compatibility.Conclusion:The oxidized bacterial cellulose/silk fibroin composite should have certain prospects of the application of smalldiameter artificial blood vessels.

关 键 词：细菌纤维素 蚕丝蛋白 复合 小口径血管 

分 类 号：R318.11[医药卫生—生物医学工程]