基于水滑石-金纳米粒子的阻抗型核酸适配体传感器的研究  

Study of Impedance Aptasensor Based on Layered Double Hydroxides-Gold Nanoparticles

作　　者：杨绍明[1] 丁素游 陈延胜[1] 张小荣[1] 郑玥[1] 郑龙珍[1] 

机构地区：[1]华东交通大学理学院化学化工系,南昌330013

出　　处：《分析化学》2016年第1期110-116,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(Nos.21465012;21065004)~~

摘　　要：在玻碳电极表面用电化学沉积法一步合成钻铝水滑石-金纳米粒子(CoAl LDH-GNPs)复合纳米材料,以复合纳米材料作为核酸适配体(Apt)的固定化基质,建立了一种高灵敏的阻抗型适配体传感器。采用扫描电镜(SEM)和能量色散谱仪(EDS)对CoAl LDH-GNPs复合纳米材料进行了表征,对电极的组装过程采用循环伏安法和电化学阻抗进行表征,对传感器的性能采用电化学阻抗进行研究。复合纳米材料CoAl LDHGNPs构筑的传感器对凝血酶(THR)具有良好的信号响应,线性相关系数R=0.995,检出限为0.3 ng/L(S/N=3),检测范围为1.0 ng/L^100μg/L。A highly sensitive impedance aptamer biosensor was fabricated on the glassy carbon electrode surface by one-step electrochemical deposition synthesis of cobalt aluminium layered double hydroxides-gold nanoparticles（CoAl LDH-GNPs） composite nanomaterials,which were used as the immobilization matrix for aptamer.The composite nanomaterials of CoAl LDH-GNPs were characterized with scanning electron microscopy（SEM） and energy dispersive spectrometer（EDS）.The electrode assembly process was monitored by the cyclic voltammetry and electrochemical impedance spectroscopy,the performance of sensor was studied by electrochemical impedance spectroscopy.The sensor fabricated by LDH-GNPs nanomaterials showed a linear relationship to thrombin from 1.0 ng/L to 100.0 μg/L with a linear correlation coefficient R=0.995 and a detection limit of 0.3 ng/L（S/N=3）.

关 键 词：水滑石复合纳米材料 阻抗型 核酸适配体传感器 凝血酶 

分 类 号：TB33[一般工业技术—材料科学与工程] TP212[自动化与计算机技术—检测技术与自动化装置]