超高效液相色谱串联质谱法同时测定马尾藻中4种内源性植物激素  

Simultaneous Determination of 4 Kind of Plant Hormones in Sargassum by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

作　　者：易勇[1,2] 唐旭[1] 林锡煌[1] 刘源森[1] 林凌[1] 徐长安[1] 

机构地区：[1]国家海洋局第三海洋研究所,厦门361005 [2]福建农林大学食品学院,福州350002

出　　处：《分析化学》2016年第1期124-130,共7页Chinese Journal of Analytical Chemistry

基　　金：海洋公益性行业专项(No.201405038);厦门南方海洋中心专项(No.14GZP004NF04);国家海洋局第三海洋研究所基本科研业务费(海三科2013005)资助

摘　　要：建立了利用超高效液相串联质谱法（UPLC-MS/MS）同时测定马尾藻中4种植物激素（吲哚乙酸、吲哚丁酸、脱落酸、玉米素）的方法。马尾藻样品经过70%甲醇提取后经PCX＋PAX固相小柱净化,使用反相C18色谱柱分离。以5 mmol/L乙酸铵（含0.01%甲酸）和甲醇作为流动相进行梯度洗脱,采用多反应离子监测模式（MRM）分析测定。在0.01-1.0μg/mL内各种植物激素的相关系数均大于0.9990。4种植物激素的回收率为84.3%-102.1%相对标准偏差为1.1%-6.4%。本方法的检出限为0.025-0.2μg/kg。A method of simultaneous determination of four kind of auxins（Indole-3-acetic acid,Indole-3-butyric acid,Abscisic acid,Zeatin） in sargassum by ultra performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS） was established.After extracted by 70%methanol,the samples were enriched by the PCX and PAX solid-phase column.Then the samples were purified by reversed phase C18 column using5 mmol/L ammonium acetate（containing 0.01%formic acid） and methanol as a mobile phase in gradient elution.On the base of multiple reaction monitoring（MRM） analysis,the results showed that the correlation coefficient for all auxins examined exceeded 0.9990 in the range of 0.01-1.0 μg/mL.The recovery was from84.3%to 102.1%,respectively.The relative standard deviation was less than 10%.Besides,the detection limit of this method was from 0.025 μg/kg to 0.2 μg/kg.

关 键 词：马尾藻 双柱联用 超高效液相色谱串联质谱 内源性植物激素 

分 类 号：Q946[生物学—植物学] O657.63[理学—分析化学]