角蛋白酶/碱性蛋白酶制备羽毛蛋白粉的工艺研究  

Technical Study on Preparation of Feather Protein Powder with Keratinase/Alkaline Protease

作　　者：郝鲁江[1] 卢晓平[1] 许艳蕊[1] 范秋苹 

机构地区：[1]齐鲁工业大学山东省微生物工程重点实验室,山东济南250353

出　　处：《中国家禽》2016年第1期32-36,共5页China Poultry

基　　金：山东省自然科学基金项目(ZR2012CM019)

摘　　要：为研究角蛋白酶/碱性蛋白酶对羽毛蛋白粉的降解效果,本试验采用不同的酶解温度、酶解液初始pH值、复酶添加量和酶解时间对羽毛蛋白粉进行酶解,并利用正交试验对酶解条件进行优化。结果表明:每克羽毛粉加入角蛋白酶0.0335 g、碱性蛋白酶0.01675 g,酶解液初始pH 11,在35℃下酶解36 h,羽毛分解率达到79.8%,酶解液可溶性蛋白含量为284.18 mg/L,酶解羽毛蛋白粉体外消化率达到30%,表明角蛋白酶/碱性蛋白酶对羽毛蛋白粉具有良好的酶解效果。To study the degradation effects of keratinase/alkaline protease on feather protein powder, different reaction temperature, initial hydrolyzate pH, complex enzyme dosage and hydrolysis time were used in hydrolysis of feather protein powder. The results suggested that feather decomposition rate was 79.8% and the soluble protein content of hydrolyzate was 284.18mg/L, enzymatic digestibility of the feather protein powder in vitro reached to 30% when feather meal was added per gram keratinase 0.0335 g,alkaline protease 0.01675 g,hydrolyzate initial pH of 11 and at 35℃ enzymatic hydrolysis of 36 h. It was concluded that the enzymatic hydrolysis effects of the keratinase/alkaline protease on feather protein powder was good.

关 键 词：角蛋白酶/碱性蛋白酶 羽毛蛋白粉 可溶性蛋白 饲料 

分 类 号：S879.9[农业科学—畜牧兽医]