氨氯地平和缬沙坦对人冠状动脉内皮细胞NADPH氧化酶亚基gp91^(phox)和p22^(phox)表达的影响  

Effect of amlodipine and valsartan on expression of the NADPH oxidase subunits gp91^(phox) and p22^(phox) in human coronary artery endothelial cell

作　　者：卢挪挪 韩卫星[1] 刘超[2] 王成[3] 吴继军[1] 

机构地区：[1]安徽医科大学第一附属医院心血管内科,合肥230032 [2]安徽医科大学基础医学院组织与胚胎学教研室,合肥230032 [3]安徽医科大学第一临床学院诊断学教研室,合肥230032

出　　处：《安徽医科大学学报》2016年第2期176-180,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81070210)

摘　　要：目的研究不同浓度的氨氯地平和缬沙坦对人冠状动脉内皮细胞(HCAEC)内尼克酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶亚基gp91phox和p22phox的影响。方法对照组:体外贴壁培养HCAEC,不进行其他干预;实验组:相同培养条件下分别加入不同浓度氨氯地平(1×10-5、1×10-6、1×10-7、1×10-8、1×10-9mol/L)和不同浓度缬沙坦(1×10-4、1×10-5、1×10-6、1×10-7、1×10-8mol/L)体外培养24 h。提取各组细胞的膜蛋白,应用Western blot法测两组细胞中p22phox和gp91phox的表达水平,对结果进行统计分析并比较。结果不同浓度氨氯地平对HCAEC内NADPH氧化酶亚基p22phox表达均有促进作用(P<0.05),高浓度氨氯地平对gp91phox有抑制作用(P<0.05),低浓度则会促进gp91phox的表达(P<0.05)。不同浓度缬沙坦对p22phox与gp91phox的表达均有显著的抑制作用(P<0.05),且有随着药物浓度增高抑制作用增强的趋势(P<0.05)。结论缬沙坦有抑制氧化应激的效力,并随浓度增高而增强,呈现浓度依赖的趋势;氨氯地平仅在高浓度时抑制部分NADPH氧化酶亚基(gp91phox)的表达,低浓度时反而可能增加氧化应激水平。Objective To study the effect of amlodipine and valsartan on expression of Nicotinamide adenine dinucleotide phosphate （NADPH） oxidase subunits gp^91phox and p22^phox in human coronary artery endothelial cells （HCAEC）. Methods HCAEC were preincubated for 24 h, and the control group remained untreated, and the test groups were treated with different concentrations of amlodipine （ 1×10^-5,1×10^-6,1×10^-7,1×10^-8, 1×10^-9 mol/L） and different concentrations of valsartan （1×10^-4, 1×10^-5, 1×10^-6, 1×10^-7, 1×10^-8 mol/L）. The NADPH oxidase subunits protein expression were assessed by Western blot analysis. Results Expressions of gp^91phox and p22^phox were significant in the experimental group and the control group. Amlodipine induced the expession of NADPH oxidase subunit p22^phox （ P 〈 0. 05 ）. High concentrations of amlodipine significantly reduced expression of gp^91phox, while low concentration promoted the expression of gp^91phox（ P 〈 0. 05 ）. p22^phox and gp^91phox were significantly inhibited by valsartan （ P 〈 0.05 ） , and the higher the concentration of valsartan, the greater the inhibiton （P 〈 0. 05 ）. Conclusion Valsartan can inhibit oxidative stress. The higher the concentration, the greater the inhibition. Amlodipine suppresses part of NADPH oxidase subunit （gp^91phox） only at high concentrations, but it may increase oxidative stress in low concentrations.

关 键 词：氨氯地平 缬沙坦 人冠状动脉内皮细胞 氧化应激 p22^phox gp91^phox 

分 类 号：R541.4[医药卫生—心血管疾病] R972.4[医药卫生—内科学]