Effects on antioxidant enzyme activities and osmolytes in Halocnemum strobilaceum under salt stress  

作　　者：TianPeng Gao Rui Guo XiangWen Fang ZhiGang Zhao GuoHua Chang YinQuan Chen Qing Zhang 

机构地区：[1]Centre of Urban Ecology and Environmental Bioteehnology,Lanzhou City University,Lanzhou,Gansu 730070,China [2]State Key Laboratory of Grassland Agro-ecosystems,Institute of Arid Agroecology,School of Life Sciences,Lanzhou University,Lanzhou,Gansu 730000,China [3]School of Chemistry and Environment Sciences,Lanzhou City University,Lanzhou,Gansu 730070,China

出　　处：《Research in Cold and Arid Regions》2016年第1期65-71,共7页寒旱区科学（英文版）

基　　金：funded by the National Natural Science Foundation of China(Grant Nos.31160118,31460162,31422011,31370402,and 30800122)

摘　　要：The seedlings of Halocnermum strobilaceum were cultivated in 0.5% hoagland nutrient solution containing 0.0%, 0.9%, 2.7% and 5.4% of NaC1 as well as composite salt （Na^＋, Ca^2＋, K^＋, Si^4＋） for 20 days; all the contents are in weight ratio. Succulent level, inorganic ions （Na^＋, K^＋）, organics such as betaine, proline, malondialdehyde, and antioxidant enzyme activities including superoxide dismutase （SOD）, catalase （CAT）, peroxidase （POD）, betaine aldehyde dehydrogenase （BADH） were measured to reveal its salt tolerance mechanism. When the composite salt concentration reaches 5.4%, SOD activity level, and MDA content is five times the control group; when it reaches 2.7%, the succulent level of seedlings, and the content ofK＋ in roots is nearly two times the NaCl treatment; the dry weight is more than three times the control group; with the NaCl treatment, MDA is three times the contrast; when the salt concentration is 2.7%, POD reaches the maximum. Results indicate that Si^4＋, K^＋, and Ca^20 from composite salt in the roots of H. strobilaceum improved the water-holding capacity. The activities of antioxidant enzyme were raised by the accumulation ofproline and betaine, which increased the salt tolerance. The absorption of K^＋ promoted the high ratio of K^＋/Na^＋ and alleviated the damage of cell membranes of H. strobilaceum, which is associated with osmotic contents such as betaine and proline.The seedlings of Halocnermum strobilaceum were cultivated in 0.5% hoagland nutrient solution containing 0.0%, 0.9%, 2.7% and 5.4% of NaC1 as well as composite salt （Na^＋, Ca^2＋, K^＋, Si^4＋） for 20 days; all the contents are in weight ratio. Succulent level, inorganic ions （Na^＋, K^＋）, organics such as betaine, proline, malondialdehyde, and antioxidant enzyme activities including superoxide dismutase （SOD）, catalase （CAT）, peroxidase （POD）, betaine aldehyde dehydrogenase （BADH） were measured to reveal its salt tolerance mechanism. When the composite salt concentration reaches 5.4%, SOD activity level, and MDA content is five times the control group; when it reaches 2.7%, the succulent level of seedlings, and the content ofK＋ in roots is nearly two times the NaCl treatment; the dry weight is more than three times the control group; with the NaCl treatment, MDA is three times the contrast; when the salt concentration is 2.7%, POD reaches the maximum. Results indicate that Si^4＋, K^＋, and Ca^20 from composite salt in the roots of H. strobilaceum improved the water-holding capacity. The activities of antioxidant enzyme were raised by the accumulation ofproline and betaine, which increased the salt tolerance. The absorption of K^＋ promoted the high ratio of K^＋/Na^＋ and alleviated the damage of cell membranes of H. strobilaceum, which is associated with osmotic contents such as betaine and proline.

关 键 词：Halocnermum strobilaceum （Pall.） salt stress SUCCULENT antioxidant enzyme activities 

分 类 号：Q945.78[生物学—植物学]