Proteomic Analysis of the Peritrophic Matrix from the Midgut of Third Instar Larvae,Musca domestica  

作　　者：Wang Yu Xiu Jiang Fan Cheng Jin Zhi Luo Man Zhao Peng Shang Xiao Li Wang Tao Wu Jian Wei 

机构地区：[1]Department of Parasitology,Guizhou Medical University,Guiyang 550004,Guizhou,China [2]guizhou provincial center for disease control and prevention,Guiyang 550004,Guizhou,Peoples R China

出　　处：《Biomedical and Environmental Sciences》2016年第1期56-65,共10页生物医学与环境科学（英文版）

基　　金：supported by National Natural Science Foundation of China(No.81360254);National Sci-Tech Support Plan of China under grant(No.2011BAC06B12);the Science and Technology Program of Guizhou Province Agricultural research Project[No.NY(2014)3054]

摘　　要：Objective To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix （PM）, a mass spectrometry approach was used to investigate the PM protein composition. Methods The PM was dissected from the midgut of the third instar larvae, and protein extracted from the PM was evaluated using SDS-PAGE. A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry （LC-MS/MS）. Results In total, 374 proteins, with molecular weights varying from 8.225, kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure. Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation. Conclusion The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.Objective To better comprehend the molecular structure and physiological function of the housefly larval peritrophic matrix （PM）, a mass spectrometry approach was used to investigate the PM protein composition. Methods The PM was dissected from the midgut of the third instar larvae, and protein extracted from the PM was evaluated using SDS-PAGE. A 1D-PAGE lane containing all protein bands was cut from top to bottom, the proteins in-gel trypsinised and analysed via shotgun liquid chromatography- tandem mass spectrometry （LC-MS/MS）. Results In total, 374 proteins, with molecular weights varying from 8.225, kD to 996.065 kD and isoelectric points ranging from 3.83 to 11.24 were successfully identified, most identified proteins were mainly related to immunity, digestion, nutrient metabolism and PM structure. Furthermore, many of these proteins were functionally associated with pattern binding, polysaccharide binding, structural constituent of peritrophic membrane and chitin binding, according to Gene Ontology annotation. Conclusion The PM protein composition, which provides a basis for further functional investigations of the identified proteins, will be useful for understanding the housefly larval gut immune system and may help to identify potential targets and exploit new bioinsecticides.

关 键 词：Musca domestica Peritrophic matrix PROTEOME 

分 类 号：R384.2[医药卫生—医学寄生虫学]