我国汉族糖尿病慢性肾脏疾病与肌肽二肽酶1基因的相关性研究  

Association analysis of polymorphism in CNDP1 gene and chronic kidney disease in type 2 diabetic Chinese Han population

作　　者：郭俊[1] 陈莉明[1] 常宝成[1] 郑妙艳[1] 温娟娟[1] 

机构地区：[1]天津医科大学代谢病医院中西医结合科,300070

出　　处：《中国糖尿病杂志》2016年第1期23-27,共5页Chinese Journal of Diabetes

基　　金：国家自然科学基金(81273915)

摘　　要：目的探讨肌肽二肽酶1(CNDP1)基因多态性与中国汉族人群糖尿病慢性肾脏疾病(CKD)的关系。方法选取健康对照者(NC)90名、T2DM患者210例和CKD患者280例(CKD组再分为GFR≥90 ml/(min·1.73 m^2)亚组105例,60 ml/(min·1.73 m^2)≤GFR<90 ml/(min·1.73 m^2)亚组84例,GFR<60 ml/(min·1.73 m^2)亚组91例),利用聚合酶链反应限制性片段长度多态性分析法(PCR-RFLP)行CNDP1 rs4892247位点多态性检测。结果 NC、T2DM和CKD组TT、CT、CC 3种基因型频率分别为66.7%、26.7%、6.7%,60.5%、31.9%、7.6%和46.8%、43.2%、10.0%;C等位基因频率分别为20.0%、23.6%、31.6%;NC组与CKD组,T2DM组与CKD组基因型和等位基因C的频率分布差异有统计学意义(P<0.05)。Logistic回归分析显示,携带C等位基因的CNDP1与CKD的发生呈正相关(OR=2.634,P<0.05)。结论 CNDP1 rs4892247基因多态性可能与中国汉族人群发生CKD发病风险增高相关。Objective To investigate the association between rs4892247 in CNDP1 gene and chronic kidney disease （CKD） in diabetes in Chinese Han population. Methods A total of 90 normal controls （NC）, 210 paitients with T2DM and 280 patients with CKD were recruited. The CKD subjects were further divided into three groups according to their estimated glomerular filtration rate（GFR）.-GFR≥90 ml/（min · 1.73 m2） subgroup （n=105） ,60 ml/（min · 1.73 m2）≤GFR〈90 ml/（min · 1.73 m2） subgroup （n=84） ,and GFR〈60 ml/（min· 1.73 m2） subgroup （n= 91）. CNDP1 gene rs4892247 genotype was tested by PCR-RFLP method. Results The frequencies of TT, CT, CC genotype were 66.7%, 26.7%, 6.7% in NC group, 60. 5%,31.9%,7.6% in T2DM group and 46.8%0,43.2%,10.0% in CKD group. There was statistically significant difference in genotypes frequency and CNDP1-C allele frequency in CKD group compare with T2DM group （P〈0.05）. Logistic regression analysis showed that the CNDP1-C allele was positively correlated with the occurrence of CKD （OR=2. 634,P〈0.05）. Conclusion CNDP1 gene rs4892247 may be associated with CKD in Chinese Han population.

关 键 词：肌肽二肽酶1 糖尿病 2型 糖尿病慢性肾脏疾病 基因多态性 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]