离子液体对玉米幼苗生长与生理生化特性的影响  

Effects of ionic liquid on the growth, physiological and biochemical characteristics of maize seedlings

作　　者：刘萍[1] 张妍[1] 丁义峰[1] 吕保鹤 范琪琪[1] 王添乐[1] 王雪瑞[1] 卢芳[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《浙江农业学报》2015年第12期2065-2070,共6页Acta Agriculturae Zhejiangensis

基　　金：河南省重点科技攻关计划项目(122102310356;142102110143)

摘　　要：探究离子液体对作物的毒害作用,可以为研究离子液体对生态环境的影响提供依据。采用水培法研究了不同浓度(0,5,10,15 mg·L^(-1))的离子液体1-己基-3-甲基咪唑溴化盐(1-hexyl-3-methyl imidazolium bromide,[C_6mim]Br)对玉米(囤玉118)幼苗生长以及叶片部分生理生化指标的影响。结果表明:在生长方面,当离子液体浓度≥10 mg·L^(-1)时,玉米幼苗株高、根长均减少,差异达到显著水平;在生理生化方面,当离子液体浓度≥10 mg·L^(-1)时,叶片中可溶性糖含量降低,达极显著水平;3个处理组的玉米幼苗叶片可溶性蛋白含量均极显著低于对照;当离子液体浓度≥10 mg·L^(-1)时,过氧化物酶(POD)活性显著低于对照;当离子液体浓度≥15 mg·L^(-1)时,超氧化物歧化酶(SOD)活性降低,超氧阴离子(O_2^(·-))产生速率升高,均达到极显著水平。证明离子液体[C_6mim]Br对主要农作物玉米具有一定的毒害作用,并且随着离子液体浓度的增加,毒害作用逐渐增大。Investigating the toxic effects of ionic liquids on crops could provide basis for the effects of ionic liquid on ecological environment. Effects of 1-hexyl-3-methyl imidazolium bromide ionic liquid （ 1-hexyl-3-methyl imidazolium bromide, [ C6mim ] Br）on the growth, physiological and biochemical characteristics of maize seedlings （Tunyu 118 ） were evaluated by hydroponics with different concentrations of [ C6mim] Br（0, 5, 10, 15 mg.L-1）. The results showed that when [ C6mim] Br concentration was greater than or equals to 10 mg. L^-1, plant height and root length of maize seedling were decreased significantly （ P 〈 0. 05 ） , and the content of soluble sugar in the leaves was decreased significantly（ P 〈 0. 01 ）. Compared with the normal control, the contents of soluble protein in the leaves was de- creased significantly whatever [ C6mim] Br concentration was set in this study. The POD activities were significantly lower than that of the control when [ C6mim ] Br concentration was greater than or equals to 10 mg· L^-1 When [ C6mim] Br concentration was≥ 15 mg.L-1, the reducing of superoxide dismutase（SOD） activity and increasing of superoxide anion（ O2- ） rate all reached significant level（ P 〈 0. 01 ） compared with the control. It proved that ionicliquid [ C6mim] Br was harmful to maize, and with the increase of ionic liquid concentration, the poison increased.

关 键 词：离子液体 玉米 毒害 可溶性蛋白 SOD POD 

分 类 号：S513[农业科学—作物学]