观赏石斛与药用石斛HPLC指纹图谱及生物活性的比较  

Comparison of HPLC Fingerprint and Bioactivity between Ornamental and Medical Dendrobium

作　　者：廖格[1] 张友源[1] 宋希强[1] 戴好富[2] 柯海丽 

机构地区：[1]海南大学园艺园林学院,海口570228 [2]中国热带农业科学院热带生物技术研究所,海口571101 [3]海南博大兰花科技有限公司,海口570311

出　　处：《热带生物学报》2015年第4期420-425,共6页Journal of Tropical Biology

基　　金：海南省产学研一体化专项(CXY20130044);国家农业公益性行业科研专项(201303117);海南省自然科学基金(314051);海南省普通高等学校研究生创新科研课题(Hys2014-08)

摘　　要：采用高效液相色谱(HPLC)法,对以春石斛(‘绿唇’、‘920’和‘31’)和秋石斛(‘红牛’、‘088’和‘T5’)为代表的观赏石斛和药用石斛(铁皮石斛、金钗石斛、鼓槌石斛和叠鞘石斛)的化学成分进行了初步分析,并对这10种石斛进行了体外降血糖和抗农业病原真菌活性测试。结果表明,10种石斛与特征指纹图谱共有模式的相似度较低,说明不同种石斛间化学成分差异较大;秋石斛‘T5’和铁皮石斛对α-葡萄糖苷酶有较好的抑制作用;春石斛‘920’和铁皮石斛对5种农业病原真菌,尤其是胶胞炭疽菌有良好的抑制活性。High Performance Liquid Chromatography （HPLC） was used to analyze the chemical components of 10 ornamental and medical Dendrobittm species and hybrids, and the component activity against agricultural pathogenic fungi and in vitro hypoglycemia were assayed by using Filter AGAR diffusion method and α-glucosidase inhibitory assay, respectively. The results showed that these ten Dendrobium species and hybrids had pretty low similarity with the shared characteristic fingerprint, which means there were obvious differences among them. Besides, the Dendrobium hybrid ＇T5＇ and D. officinale had preferable inhibitory effect on α-glucosidase, and the hybrid ＇920＇ and D. had the inhibitory activity against 5 especially Colletotrichum gloeosporiodes. species of agricultural pathogenic fungi,

关 键 词：观赏石斛 HPLC 化学成分 生物活性 

分 类 号：S682.31[农业科学—观赏园艺]