Identification of Variable-Number Tandem-Repeat(VNTR)Sequences in Acinetobacter pittii and Development of an Optimized Multiple-Locus VNTR Analysis Typing Scheme  

作　　者：HU Yuan LI Bo Qing JIN Da Zhi HE Li Hua TAO Xiao Xia ZHANG Jian Zhong 

机构地区：[1]State Key Laboratory of Infectious Disease Prevention and Control,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention [2]Binzhou Medical College [3]Zhejiang Provincial Center for Disease Control and Prevention

出　　处：《Biomedical and Environmental Sciences》2015年第12期855-863,共9页生物医学与环境科学（英文版）

基　　金：supported by the Major Infectious Diseases Such as AIDS and Viral Hepatitis Prevention and Control technology major projects(grants 2013ZX-100040101,2013ZX10004805-005);the key projects of state key laboratory of infectious disease prevention and control(grants 2014SKLID102)

摘　　要：Objective To develop a multiple-locus variable-number tandem-repeat(VNTR)analysis(MLVA)assay for Acinetobacter pittii typing.Methods Polymorphic VNTRs were searched by Tandem Repeats Finder.The distribution and polymorphism of each VNTR locus were analyzed in all the A.pittii genomes deposited in the NCBI genome database by BLAST and were evaluated with a collection of 20 well-characterized clinical A.pittii strains and one reference strain.The MLVA assay was compared with pulsed-field gel electrophoresis(PFGE)for discriminating A.pittii isolates.Results Ten VNTR loci were identified upon bioinformatic screening of A.pittii genomes,but only five of them showed full amplifiability and good polymorphism.Therefore,an MLVA assay composed of five VNTR loci was developed.The typeability,reproducibility,stability,discriminatory power,and epidemiological concordance were excellent.Compared with PFGE,the new optimized MLVA typing scheme provided the same and even greater discrimination.Conclusion Compared with PFGE,MLVA typing is a faster and more standardized alternative for studying the genetic relatedness of A.pittii isolates in disease surveillance and outbreak investigation.Objective To develop a multiple-locus variable-number tandem-repeat(VNTR)analysis(MLVA)assay for Acinetobacter pittii typing.Methods Polymorphic VNTRs were searched by Tandem Repeats Finder.The distribution and polymorphism of each VNTR locus were analyzed in all the A.pittii genomes deposited in the NCBI genome database by BLAST and were evaluated with a collection of 20 well-characterized clinical A.pittii strains and one reference strain.The MLVA assay was compared with pulsed-field gel electrophoresis(PFGE)for discriminating A.pittii isolates.Results Ten VNTR loci were identified upon bioinformatic screening of A.pittii genomes,but only five of them showed full amplifiability and good polymorphism.Therefore,an MLVA assay composed of five VNTR loci was developed.The typeability,reproducibility,stability,discriminatory power,and epidemiological concordance were excellent.Compared with PFGE,the new optimized MLVA typing scheme provided the same and even greater discrimination.Conclusion Compared with PFGE,MLVA typing is a faster and more standardized alternative for studying the genetic relatedness of A.pittii isolates in disease surveillance and outbreak investigation.

关 键 词：A.pittii MLVA typing VNTR 

分 类 号：R378[医药卫生—病原生物学]