慢性低钾因素对家兔心肌和骨骼肌组织K＋摄取率的影响  

Effect of chronic low potassium on K＋ uptake rate in myocardium and skeletal muscle of rabbits

作　　者：曹瑞旗[1] 李雪卿[1] 吴文浩[1] 马淑贤[1] 杨运亮[2] 董振明[1] 

机构地区：[1]河北医科大学第二医院麻醉科,石家庄市050000 [2]石家庄市第一医院麻醉科,石家庄市050000

出　　处：《中华麻醉学杂志》2015年第12期1498-1500,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价慢性低钾因素对家兔心肌和骨骼肌组织钾离子（K＋）摄取率的影响。方法清洁级成年雄性家兔32只，12～14周龄，体重2．O～2．7kg，采用随机数字表法分为4组（n=8）：正常饲喂组（N组）、低钾饲喂组（L组）、补钾对照组（SC组）和补钾实验组（SE组）。N组和SC组喂食正常饲料，L组和SE组喂食低钾饲料15d，随后SE组和SC组静脉输注氯化钾（KC1）0．5mol／L，起始速率60μm01·kg-1·min-1，每隔5min采集左耳中央动脉血样0．5ml，测定血浆K＋浓度后调整KC1输注速率，至血浆K＋浓度达5．5mmol／L时稳定1h停止输注。记录KC1输注总量，取心脏和比目鱼肌组织标本测定K＋含量，计算K＋摄取量和摄取率。结果与N组比较，L组家兔血浆K＋浓度、心肌组织和比目鱼肌组织K＋含量降低（P〈0．05）。与SC组比较，SE组KCI输注总量增加，心肌组织和比目鱼肌组织K＋摄取量增加，摄取率升高（P〈O．05）。结论慢性低钾因素可增加家兔心肌和骨骼肌组织K＋摄取率。Objective To evaluate the effect of chronic low potassium on K＋ uptake rate in the my- ocardium and skeletal muscle of rabbits. Methods Thirty-two adult male rabbits, aged 12- 14 weeks, weighing 2.0-2.7 kg, were randomly divided into 4 groups （n = 8 each） using a random number table： normal feeding group （group N）, low potassium feeding group （group L）, potassium supplementation con- trol group （group SC） and potassium supplementation experimental group （group SE）. N and SC groups were given a normal diet only, and L and SE groups were fed with a low potassium diet for 15 days. Potassi- um chloride （KC1） 0. 5 mol/L was then infused intravenously at the initial rate of 60 μmol · kg-1 · min-1 in SE and SC groups. Blood samples were obtained from the central artery of the left ear every 5 min for meas- urement of plasma K＋ concentrations. The infusion rat of KC1 was then adjusted until the plasma K＋ concen- tration reached 5.5 mmol/L and maintained at this level for 1 h, and then infusion was stopped. The total volume of KCI infused was recorded. The hearts and soleus muscle of animals were excised for determination of K＋content. K＋ uptake and uptake rate were calculated. Results Compared with N group, the plasma K＋ concentration, and K＋content in the myocardium and soleus muscle were significantly decreased in group L （P〈0.05）. Compared with SC group, the total volume of KC1 infused, and K＋ uptake and uptake rate in the myocardium and soleus muscle were significantly increased in group SE （P〈0. 05）. Conclusion Chro- nic hypokalaemia can increase K＋ uptake rate in the myocardium and skeletal muscle of rabbits.

关 键 词：低钾血症 心肌 肌 骨骼 

分 类 号：R614[医药卫生—麻醉学]