刺参肠道及养殖池塘底泥微生物多样性的比较研究  

A Comparative Study of Microbial Diversity in the Intestine of Sea Cucumber and Breeding Pond Sediment

作　　者：夏海峰[1] 杜宗军[1] 陈冠军[1] 

机构地区：[1]山东大学(威海)海洋学院,山东威海264209

出　　处：《海洋湖沼通报》2015年第4期105-110,共6页Transactions of Oceanology and Limnology

基　　金：国家科技重大专项(2013ZX10004217)资助

摘　　要：刺参养殖作为北方环渤海地区新兴的经济产业,池塘养殖是其主要的养殖模式,而传统养殖模式中其生长环境易受外界影响,池塘底泥和刺参消化道中菌群的变化易引起刺参病害的发生,本文旨在通过研究不同季节刺参肠道及刺参养殖池底泥中菌系多样性及变化为深入了解刺参池塘养殖系统微生物的结构与功能提供参考。运用纯培养方法和16SrRNA基因序列分析,在1年中分4次取样,从刺参肠道和刺参养殖池底泥中分离出共151株海洋菌株,其中肠道菌72株,养殖池塘底泥79株,发现新菌种5株,拮抗菌株1株,通过构建系统发育树对刺参肠道及养殖环境底泥多样性进行研究。结果显示:分离的菌株分属海王星菌属(Neptuniibacter)、假交替单胞菌属(Pseudoalteromonas)、迪茨氏菌属(Dietzia)、铁单胞菌属(Ferrimonas)、需盐杆菌属(Salegentibacter)等31个属;通过TCBS培养基筛选后发现弧菌属(Vibrio)在刺参肠道中有32株,底泥中28株,是刺参肠道和底泥环境中的第一大优势菌属,其数量随着时间变化呈上升趋势。Sea cucumber breeding is the new economic industry in the bohai area.Pond culture is the main mode of farming,but it＇s easily affected by the change of the environment,the change of strains in the sediment and the digestive tract of sea cucumber is easy to cause the disease,we aim to study their change in different season to provide reference for understanding the structure and function of microorganisms in this ecosystem.We sampled sea cucumber and the pond sediment 4times in a year,a total of151 strains of marine bacteria were isolated from them.These strains belong to 31 genera,72strains were found from the intestine of sea cucumber,and the others were isolated from the sediment.Vibrio is the most dominant genus in this ecosystem.

关 键 词：刺参 肠道 底泥 细菌生物多样性 

分 类 号：Q939.1[生物学—微生物学]