网状原角藻化感作用的初步研究  

A Preliminary Study on the Allelopathy of Protoceratium reticulatum

作　　者：庞敏[1] 屈佩[2] 徐金涛[3] 孙萍[1] 胡小颖[1] 张学雷[1] 王宗灵[1] 

机构地区：[1]国家海洋局第一海洋研究所,山东青岛266061 [2]中国海洋大学海洋生命学院,山东青岛266061 [3]国家海洋局秦皇岛海洋环境监测中心站,河北秦皇岛066002

出　　处：《海洋湖沼通报》2015年第4期120-126,共7页Transactions of Oceanology and Limnology

基　　金：国家海洋局近岸海域生态环境重点实验室开放基金项目(201106);国家海洋局青年海洋科学基金项目(2012123);国家海洋局第一海洋研究所基本科研业务费专项基金项目(GY02-2011T07)资助;青岛市科技计划基础研究项目(13-1-4-234-jch)

摘　　要：为明确海洋甲藻——网状原角藻(Protoceratium reticulatum)对海洋微藻的化感作用,揭示其化感物质作为植物源抑藻剂的开发潜能,本文探讨了网状原角藻藻细胞和无细胞滤液对几种海洋微藻——东海原甲藻(Prorocentrum donghaiense)、拟旋链角毛藻(Chaetoceros pseudocurvisetus)、米氏凯伦藻(Karenia mikimotoi)和赤潮异弯藻(Heterosigma akashiwo)生长的影响,并进一步选取东海原甲藻和赤潮异弯藻作为受体藻种,以塔玛亚历山大藻(Alexandrium tamarense)作为阳性对照,比较了2种甲藻的藻细胞培养液和无细胞滤液对东海原甲藻、赤潮异弯藻生长的影响差异。通过比较发现,网状原角藻的无细胞滤液比塔玛亚历山大藻能更明显的抑制东海原甲藻和赤潮异弯藻的生长,尤其是对东海原甲藻,基本阻断其生长。To investigate the allelopathic activity of Protoceratium reticulatum,experiments were designed to study the allelopathic effect of cells and cell free used medium of Protoceratium reticulatum on the growth of other marine microalgae,such as Prorocentrum donghaiense,Chaetoceros pseudocurvisetus,Karenia mikimotoi and Heterosigma akashiwo.In addition,Alexandrium tamarense was chosen as apositive control and P.donghaiense,H.akashiwo were chosen as the target species in order to estimate the strength of allelopathic activity of P.reticulatum.The results showed that the growth of P.donghaiense and H.akashiwo was affected obviously by donor species each when cocultured.The cell free used medium of P.reticulatum had a stronger influence on the growth of the two target species,especially on that of P.donghaiense as its growth was almost completely blocked.

关 键 词：化感作用 甲藻 网状原角藻 塔玛亚历山大藻 东海原甲藻 

分 类 号：Q175[生物学—水生生物学]