水稻无选择标记Xa21转基因系CX8621的获得与遗传分析  

Generation and Genetic Analysis of Xa21 Transgenic Rice Line CX8621 Without Selectable Markers

作　　者：夏志辉[1,2] 刘鹏程[1] 高利芬[1] 刘栋峰[1] 姜丽[1] 江光怀[1] 郭乐群[1] 翟文学[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100101 [2]海南省热带生物资源可持续利用重点实验室/海南大学农学院,海口570228

出　　处：《中国水稻科学》2016年第1期10-16,共7页Chinese Journal of Rice Science

基　　金：国家转基因生物新品种培育重大专项(2008/2014ZX08001-002);中国科学院知识创新方向项目(KSCX2-EWN-01)

摘　　要：无选择标记和转基因稳定遗传是转基因作物安全应用的基本要求。按照我国转基因作物安全评价指南,利用双右边界T-DNA载体系统将广谱抗白叶枯病基因Xa21转入水稻恢复系明恢86。通过抗性鉴定、PCR分析和Southern杂交,跟踪了外源Xa21基因在转基因后代植株的遗传,并在T3代获得了无选择标记和载体骨架序列的单拷贝抗病纯合系CX8621。多年的观察和鉴定表明转基因恢复系CX8621具有稳定的白叶枯病抗性。目前CX8621已经稳定遗传至T16,并已通过小规模的田间试验(环境释放)和大规模的田间试验(生产性试验)的安全评价。Selectable marker-free and stable inheritance are the basic requirements for safe application of transgenic crops. Following the regulations approved by the National Committee for Transgenic Biosafety, the wide spectrum bacterial blight resistance gene Xa21 was transferred into a widely used restorer line Minghui 86 by using a double right-borders T-DNA vector through Agrobacterium tumefaciens-mediated system. The transgene Xa21 was confirmed by resistance identification PCR analysis and Southern blot in the progenies. The single-copy homozygous transgenic line CX8621 without seleetable marker and vector backbone sequences was obtained from T3 generation. The transgenic restorer line CX8621 has stable bacterial blight resistance with years of observation and identification. Up to now, CX8621 has self-fertilized to 16th generation with stable inheritance of transgene Xa21 and passed the bio-safety evaluation of small and large scale of field tests （environmental release and productive test）.

关 键 词：转基因水稻 白叶枯病 XA21 无选择标记 稳定遗传 

分 类 号：S511.21[农业科学—作物学]