水稻剑叶角度qFla-8-2位点的精细定位  

Fine Mapping of qFla-8-2 for Flag Leaf Angle in Rice

作　　者：朱长丰[1] 梁利君[1] 曾思远[1] 李天伟[1] 董冠杉 洪德林[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,南京210095

出　　处：《中国水稻科学》2016年第1期27-34,共8页Chinese Journal of Rice Science

基　　金：国家863计划资助项目(2010AA101301);高等学校博士学科点专项科研基金资助项目(20130097110001);中央高校基本科研业务费专项资金项目(KYZ201202-9)

摘　　要：为更好地理解水稻大剑叶角的分子遗传机制,对已初定位的控制剑叶角度的qFla-8进行精细定位和候选基因预测。利用以863B为受体亲本、A7444为供体亲本衍生的BC3F3群体中仅在目标基因区段杂合的172个单株自交构建的次级分离群体,对qFla-8所在的RM6215-RM8265区间9个SSR标记的基因型进行鉴定,结合表型数据进一步缩短qFla-8所在染色体区段。结果发现qFla-8位点所在染色体区段存在两个紧密连锁的位点qFla-8-1和qFla-8-2。其中,qFla-8-1位于RM6215和RM3153之间,可解释22.33%的表型变异;qFla-8-2位于RM1309和RM3491之间,可解释23.81%的表型变异。进一步对加性效应较大的qFla-8-2精细定位,通过开发插入缺失(InDel)分子标记,将qFla-8-2位点限定于InDel标记Z7和SSR标记RM23071之间,该区间的物理距离为67 kb。该区段包含3个预测基因Os08g0408200,Os08g0408300和Os08g0408500,分别编码功能类似GAMYB的WD40结构域蛋白、假定蛋白以及类APETALA2蛋白。To better understand the genetic mechanism regulating large rice flag leaf angle, qFla-8 detected previously was further fine-mapped and the candidate genes were predicted. Using a secondary segregating population, composed of 172 selling individual plants with heterozygous region of target gene merely in BC3 F3 population derived from the backcross between 863B （recipient parent） and A7444 （donor parent）, genotypes of nine SSR markers in the interval of RM6215- RM8265 containing qFla-8 were identified. Combining the phenotypic data, chromosome segment containing qFla-8 was further narrowed and there are two closely linked loci, qfla-8-1 and qfla-8-2.qFla-8-1 was localized between RM6215 and RM3153, explaining 22.33% of phenotypic variation, and qFla-8-2 was localized between RM1309 and RM3491, explaining 23.81% of phenotypic variation. By developing insertion-deletion （InDel） markers, qFla-8-2 with higher additive effect was finally limited to 67 kb region between InDel marker Z7 and SSR marker RM23071, which contains three predicted genes, Os08g0408200 encoding WD40 domain containing protein similar to GAMYB-binding protein, Os08g0408300 encoding hypothetical protein and Os08g0408500 encoding APETALA2-1ike protein.

关 键 词：水稻 剑叶角度 QTL 精细定位 

分 类 号：S511[农业科学—作物学]