检测特异抗体胶体金免疫层析试条诊断内脏利什曼病患者现场评价  

Evaluation of colloid gold labeled immunochromatographic strip test for rapid detection of canine Leishmanial infection in China

作　　者：冯晓平[1] 高春花[1] 石锋[1] 杨玥涛[1] 汪俊云[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,国家级热带病国际联合研究中心,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海200025

出　　处：《中国人兽共患病学报》2016年第1期24-27,共4页Chinese Journal of Zoonoses

基　　金：国家科技重大专项(No.2012ZX10004-220和2012ZX10004-201)~~

摘　　要：目的在现场评价检测内脏利什曼病特异抗体的胶体金免疫层析试条诊断内脏利什曼病患者的效果。方法2013年采集动物源型内脏利什曼病流行区(四川省、甘肃省)和人源型内脏利什曼病流行区(新疆喀什市)疾病预防控制中心门诊就诊的病人血样和骨髓样本,用镜检法、内脏利什曼病试条和rK39试条进行平行测试,以镜检法为金标准,比较两种试条法检测的敏感性和特异性有无差异。结果内脏利什曼病试条和rK39试条检测镜检确诊的97例内脏利什曼病患者的敏感性分别为98.87%(96/97),97.94%(95/97),二者无统计学差异(χ2=0.34,P>0.05)。两种试条检测非内脏利什曼病病例145例,均有2例假阳性反应,特异性为98.62(143/145)。对来自动物源型内脏利什曼病流行区和人源型内脏利什曼病流行区的内脏利什曼病患者分别统计阳性率,结果显示内脏利什曼病试条和rK39试条法检测动物源型和人源型流行区的内脏利什曼病患者血样之间阳性率的差异均无统计学意义(χ2=0.22/0.01,P>0.05)。两种试条法检测动物源型和人源型内脏利什曼病流行区非内脏利什曼病患者血样之间特异性的差异也均无统计学意义(χ2=0.29,P>0.05)。结论快速检测内脏利什曼病的胶体金免疫层析试条适用于检测动物源型内脏利什曼病流行区患者血样中的特异性抗体。We evaluated a colloid gold immunochromatographic strip test（ICT） developed by our laboratory for detection of human visceral leishmaniasis in field. The human blood and bone marrow samples from the zoonotic visceral leishmaniasis endemic areas（Sichun and Gansu provinces） and a anthroponotic visceral leishmaniasis endemic area（Kashi, Xinjiang） of suspected visceral leishmaniasis were collected and subjected to be detected by the microscopy, ICT and rK39. Detection results of the ICT were compared with that of the rK39 and then compared with microscopy findings（taken as a golden standard）. It was found that the positive detection rates of ICT and rK39 were 98.87 % （96/97） and 97.94 % （95/97）, respectively. There was no statistical difference in the sensitivity between ICT and rK39 （Z2 = 0.34, P〉0.05）. Detection on 145 blood samples of patients with non-visceral leishmaniasis showed both specificities of 98. 62 （143/145） by the ICT and rK39. There was no statistical difference in the sensitivity between samples from the zoonotic visceral leishmaniasis endemic areas and the anthroponotic vis- ceral leishrnaniasis endemic area by the ICT（x2=0.22, P〉0.05）. It is evident that the ICT for detection the leishmanial spe- cific antibody of human peripheral blood specimens is proved to be a reliable method.

关 键 词：内脏利什曼病 免疫层析试条 现场评价 

分 类 号：R531.6[医药卫生—内科学]