正交试验法优选大蝎子草总黄酮提取工艺  

Optimization of Extraction Tecnique of Total Flavonoids in Girardinia diversifolia by Orthogonal Test

作　　者：徐旖旎[1,2] 姜丰[1,2] 李守巧 周雪 刘文炜[1,2] 沈祥春[2] 张彦燕[1,2] 

机构地区：[1]贵州医科大学基础医学院,贵州贵阳550004 [2]贵州医科大学天然药物优效利用重点实验室,贵州贵阳550004 [3]临沭县人民医院,山东临沭276700

出　　处：《贵阳医学院学报》2016年第1期41-44,共4页Journal of Guiyang Medical College

基　　金：贵阳市科技局现代药业计划(2012204-41);贵州省中医药管理局(QZYY2012-46);贵州省中医药管理局课题(QZYY2019-92);贵阳市科技局大学生创新课题(黔科合同2012-2);贵州省高等教育科技创新团队[黔教合人才团队字(2014)31];贵州省高等学校创新能力提升计划[黔教合协同创新字(2013)04];贵州省高层次创新型人才[黔科合人才(2015)4029];贵州省科技创新团队[黔科合人才团队(2015)4025号]

摘　　要：目的:优选大蝎子草(HGD)总黄酮最佳提取工艺。方法:以加水量(A),提取次数(B),提取时间(C)为影响因素,采用正交试验方案L9(34)进行HGD总黄酮的提取,紫外分光光度法在波长269 nm处测定吸光度,以浸膏收率、总黄酮含量为考察指标,进行综合加权评分,确定HGD总黄酮最佳提取工艺。结果:最佳水提取工艺为A2B3C3,即18倍加水量、提取3次、每次提取3 h时可获得较高的浸膏收率及总黄酮含量;加权评分方差分析结果表明:因素B及因素C对总黄酮提取量有显著影响。结论:利用正交试验设计方法对HGB总黄酮提取工艺进行优化,为该药材的深入研发提供一定的参考。Objective： To optimize the technique to extract total flavonoids （TFA） in Girardinia diversifolia （HGD）. Methods： The amount of water （ A）, extracting frequency （ B ） and time （ C ） were used as influencing factors, extracting HGD TFA with orthogonal test L9 （34 ）. Determination of absor- bance at the wavelength of 269 nm by UV Spectrophotometry with extract yield, content of TFA as inspection indexes. Then comprehensive weighted scoring was applied to determine optimized HGD THA extraction technique. Result： The best technique was A2B3C3, added 18 times volume of water and extracted for 3 times, 3 hours each time in order to acquire relatively high extract yield and TFA con- tent. Analysis of weighted scoring variance indicated that factor B and C had significant influence on TFA extraction amount. Conclusion： Optimization of extraction technique by orthogonal test can reduce the time of experiment.

关 键 词：大蝎子草 黄酮类 正交实验 工艺学 制药 

分 类 号：R284.2[医药卫生—中药学]