TO901317促进人乳腺癌细胞凋亡的研究  

TO901317 induces the apoptosis of MCF-7 and MDA-MB-231 human breast cancer cells

作　　者：涂剑[1] 刘晓旺[1] 丁维珂 余平[1] 陆凯强 陈霄霄[1] 陈雪艳[1] 周志刚[2] 

机构地区：[1]南华大学药物药理研究所湖南省分子靶标新药研究协同创新中心 [2]南华大学第一附属医院

出　　处：《中国临床药理学与治疗学》2015年第12期1340-1347,共8页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金(81541163);湖南省自然科学基金(2015JJ3101);国家级大学生创新创业训练计划项目(201410555008);湖南省大学生创新性课题(2014-234);衡阳市科技局课题(2013KJ22);"湖南省分子靶标新药研究协同创新中心"培育项目(2014-405);湖南省"十二五"重点学科建设项目共同资助

摘　　要：目的:研究肝X受体激动剂TO901317对人乳腺癌细胞凋亡的影响。方法:不同浓度(0,10,20,40μmol/L)TO901317处理MCF-7和MDA-MB-231两种人乳腺癌细胞不同时间(0,12,24,48 h),用MTT法检测细胞活力,Hoechst33342染色及流式Annexin V-FITC/PI双染法检测细胞凋亡;Western blot进一步检测细胞凋亡相关蛋白Bcl-2、Bax、Caspase 3和cleaved-Caspase 3等的表达。结果:随着TO901317处理浓度的增加和时间的延长,对细胞活力的抑制作用逐渐增强,凋亡现象逐渐明显。进一步通过Western blot检测发现,TO901317可下调Bcl-2蛋白表达,促使凋亡蛋白Bax和cleaved-Caspase 3表达增多,进一步证实TO901317促进两种乳腺癌细胞凋亡。结论:TO901317能促进MCF-7和MDA-MB-231两种人乳腺癌细胞凋亡。AIM： The apoptotic effect of TO901317 on human breast cancer cells was researched so as to provide a theoretical basis for diagnosis and therapy for breast cancer. METHODS：MCF-7 and MDA-MB-231 cells were treated with different concentration（ 0,10,20,40 μmol / L） of TO901317 for different time（ 0,12,24,48 h）.Then,the cell viability was detected by MTT assay,the cell apoptosis was determined with annexin V /Propidium iodide staining and Hoechst 33342 staining analysis,and the expression of apoptosis-related proteins such as Bcl-2, Bax, Caspase 3 and cleaved-Caspase 3 were determined by western blot.RESULTS： Firstly,TO901317 inhibited the viability of MCF-7 and MDA-MB-231 human breast cancer cells and induced the cell apoptosis in a dose- and time-dependent manner. Then,the expression of apoptosis-related proteins like Bax and cleavedCaspase 3 were up-regulated but Bcl-2 was downregulated by western blot. CONCLUSION：TO901317 could induce the apoptosis of MCF-7 and MDA-MB-231 human breast cancer cells.

关 键 词：TO901317 人乳腺癌细胞 细胞凋亡 

分 类 号：R965.2[医药卫生—药理学]