傣药咪多领(云南琵琶甲)对兔耳增生性瘢痕TGF-β1 mRNA表达的影响  

Effect of Miduoling( Yunnan Pipa A) on the TGF- β1 mRNA Expression of Rabbit Ear Hypertrophic Scars

作　　者：赵文斌[1] 李娅[2] 赵川[2] 黄玉芍[1] 欧阳晓勇[1] 黄虹[1] 

机构地区：[1]云南省中医医院,云南昆明650021 [2]昆明医科大学第一附属医院,云南昆明650041

出　　处：《云南中医中药杂志》2016年第1期71-73,共3页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：云南省应用基础研究计划面上项目(NO:2011FZ271)

摘　　要：目的观察傣药咪多领(云南琵琶甲)提取物制剂对兔耳增生性瘢痕组织TGF-β1 mRNA表达的影响。方法建立兔耳增生性瘢痕动物模型,术后28天将模型随机分为实验组、阳性药物(康瑞保)对照组和空白对照组,分别外用琵琶甲制剂、康瑞保或生理盐水,4周后取材观察各组瘢痕组织形态,采用RT—PCR方法检测转化生长因子TGF-β1mRNA的表达。结果实验组与空白对照组相比,TGF-β1 mRNA表达量明显降低(P<0.01);实验组与阳性对照组相比,TGF-β1 mRNA表达量无差异(P>0.05)。结论傣药咪多领(云南琵琶甲)可以减少增生性瘢痕TGF-β1 mRNA的表达。Objective： To observe the effect of Miduoling （Yunnan Pipa A） extract preparation on TGF - βl mRNA expression of rabbit ear hypertrophic scar tissue. Methods ： Animal models of rabbit ear hypertrophic scars were made. After 28 days, the models were randomly divided into an experimental group, a positive drug （contractubex） group and a blank control group. They were respectively given topical use of Pipa A, Contractubex and saline. The scar tissue forms of each group were harvested and observed after four weeks. RT - PCR method was used to detect the TGF- βlmRNA expression of transforming growth factors. Results： Compared with the control group, the TGF - βl mRNA expression of the experimental group was significantly lower （P 〈 0. 01 ）. When compared with the positive group, the TGF- βl mRNA expression of the experimental group was no difference （P 〉 0.05 ）. Conclusion： Miduoling （Yunnan Pipa A） can re- duce the TGF - βl mRNA expression of hypertrophic scars.

关 键 词：琵琶甲 增生性瘢痕 动物模型 TGF-Β1 RT-PCR 

分 类 号：R29[医药卫生—民族医学]