细菌显像剂^(18)F-FDS的合成及动物显像应用研究  

Radiosynthesis of Infection Tracer ^(18)F-FDS and Animal Imaging

作　　者：邢海群[1] 朱文佳[1] 蔡炯[1] 张迎强[1] 要少波[1] 霍力[1] 李方[1] 

机构地区：[1]中国医学科学院北京协和医院核医学科,100730

出　　处：《医学研究杂志》2016年第1期51-54,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(面上项目)(81571712)

摘　　要：目的探讨细菌显像剂2-脱氧-2-氟山梨醇(2-deoxy-2-[^(18)F]-fluorosorbitol,^(18)F-FDS)的合成过程、正常小鼠显像并计算内照射剂量,比较小鼠模型中大肠杆菌感染病灶及肿瘤病灶对FDS的摄取情况。方法^(18)F-FDS由氟多功能模块经"一锅法"由PET常用显像剂2-氟-2脱氧葡萄糖(18F-fluorodeoxyglucose,1^(18)F-FDG)经Na BH4还原制备;实验组感染小鼠及荷瘤鼠尾静脉注射^(18)F-FDS后60min进行micro PET显像,通过Inveron Research软件测量病灶靶/本底比值;对照组注射^(18)F-FDG,同样方法测量病灶靶/本底比值进行对照分析。结果^(18)F-FDS放化收率为80%±5%(n=8,未进行时间衰减校正),合成时间25min,放化纯度>99%。正常小鼠尾静脉注射^(18)F-FDS后micro PET显像提示示踪剂从泌尿系统排泄;背景组织肺及脑内的非特异性放射性摄取较低;大肠杆菌感染小鼠micro PET显像,感染灶有较高的感染灶/肌肉比值,荷瘤鼠显像示^(18)FFDS肿瘤组织靶/本底比值明显低于^(18)F-FDG。结论^(18)F-FDS放化合成方法操作简单,收率高,细菌感染灶有特异性摄取,在分辨感染和肿瘤上具有应用前景。Objective To synthesis ^18F - FDS from ^18F - FDG, calculate internal radiation dose in normal mice imaging, look for the difference of FDS accumulated in E. coli infection mouse model and in tumor lesions. Methods ^18F - FDS was synthesized on multifunctional synthesis module from ^18F- FDG with sodium borohydride reduction by ＇One -pot reaction＇. In test group, ^18F -FDS was injected into infection mice model and tumor mice model via tail vein for microPET imaging after 60min. T/B values of ^18F - FDS were measured and analyzed by Inveron Research software, ^18F - FDG was injected into model mice in control group and T/B values were measured as describe above for comparison. Results It took 25 min to complete ^18F - FDS synthesis. The radiochemical yield and radiochemistry purity of ^18F - FDS was 80% ± 5% , and over 99% respectively. MicroPET imaging data with normal mouse suggested that ^18F - FDS was cleared from kidneys. The nonspecific uptake of^18F -FDS in background tissue such as lung, brain was very low. MicroPET imaging data showed that E. coli infected loci in mouse model had high T/B ratio, while tumor lesions had much lower T/B ratio. Conclusion ^18F - FDS is easy to be prepared with high yield and can be used to trace bacteria infected loci specifically. It is a promising radiotracer for differentiated diagnosis of bacteria infection from tumor.

关 键 词：感染 ^18F—FDS 大肠杆菌 肿瘤 放化标记 

分 类 号：R445.5[医药卫生—影像医学与核医学]