胚胎大鼠背根神经节神经元的原代培养  

Primary Culture of Embryonic Dorsal Root Ganglion Neurons in Rat

作　　者：孙青[1] 梁晓春[1] 张宏[2] 

机构地区：[1]中国医学科学院/北京协和医学院北京协和医院中医科,100730 [2]中国医学科学院/北京协和医学院基础医学研究所细胞中心,100005

出　　处：《医学研究杂志》2016年第1期66-68,73,共4页Journal of Medical Research

基　　金：北京市自然科学基金资助项目(7082077)

摘　　要：目的原代培养胚胎大鼠背根神经节神经元(DRGn),并观察其生长特性。方法取E15 SD胎鼠的背根神经节,用胰蛋白酶消化法分离成单细胞,通过密度梯度离心法和差速贴壁法进行分离纯化,在无血清培养基中培养,用抗神经元特异性烯醇化酶(NSE)抗体鉴定神经元。结果纯化培养神经元生长状态良好,纯度可达93%左右。结论该方法具有较好的可操作性和可重复性,用于DRGn的相关实验研究。Objective To improve the methods for primary culture of embryonic dorsal root ganglion（DRG） neurons in rats. Methods DRGs were harvested from embryonic day 15 Sprague Dawley rats and dissociated in trypsin, purified by density gradient centrifugatiou and differential attachment technique, then cultured in neurobasal medium, and immunofluorescent method with neuronal specific enolase（NSE） antibody was used to identify them. Results DRG neurons survived and grew nicely in the medium, and the purified rate of DRG neurons could reach to around 93%. Conclusion It was a simple and reliable method to culture DRG neurons which can be used for the related researches.

关 键 词：背根神经节神经元 原代培养 胚胎大鼠 

分 类 号：R3[医药卫生—基础医学]