浒苔藻体叶绿素提取方法的比较  

A comparative study of the different extraction techniques about chlorophyll concentration of Ulva prolifera

作　　者：刘湘庆[1,2,3] 姜美洁[3] 刘璐[3] 李艳[2,3] 张学雷[2,3] 李瑞香[2,3] 王宗灵[1,2,3] 

机构地区：[1]中国海洋大学环境科学与工程学院,山东青岛266100 [2]青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室,山东青岛266071 [3]国家海洋局第一海洋研究所,山东青岛266061

出　　处：《海洋环境科学》2016年第1期144-148,共5页Marine Environmental Science

基　　金：国家自然科学基金项目(41276119);山东海洋科学研究中心联合基金项目(U1406403)资助;国家自然科学青年基金项目(41306171);海洋公益性行业科研专项项目(201105023-2)

摘　　要：为了确定一种快速准确测定浒苔藻体叶绿素含量的方法,利用分光光度计法比较了两种不同的提取方法及4种提取剂测定叶绿素的效果,并分析了叶绿素在4种提取剂中的稳定性。结果表明:两种提取方法下4种不同提取剂的吸收光谱基本相似,可以用Arnon法的计算公式来计算叶绿素含量。其中,研磨法对浒苔叶绿素的提取效果较浸提法提取速度更快,便于快速测定藻体叶绿素含量。在4种提取剂中,采用95%乙醇提取浒苔叶绿素速度更快,但提取的总量少,稳定性低。In order to rapid and accurate determine chlorophyll content of Ulva prolifera ,we compared the efficiency of the chlorophyll extractives using spectrophotometer with two different techniques and four extracts, and studied the sta- bility of chlorophyll extractives in four extracts. The results indicated that chlorophyll absorption spectrum was similar in four extracts, accorded with the formula of Aruon to estimate the chlorophyll content. In addition, rubbing technique was better than marinating technique in efficiency of chlorophyll extractives, which was good for rapid determination of algal chlorophyll content. 95% ethanol was better than other extracts, but the chlorophyll concentration was less and the stability was worse.

关 键 词：叶绿素 浒苔 研磨法 浸提法 

分 类 号：O331[理学—一般力学与力学基础]