两种海洋寡糖对仿刺参免疫活性的影响  

Influnce of two marine oligosaccharides on the immune activity of juvenile sea cucumber

作　　者：刘美思[1,2] 程立坤[2] 罗希[2] 许青松[2] 王秀武[1] 尹恒[2] 

机构地区：[1]辽宁师范大学生命科学学院,大连116021 [2]中国科学院大连化学物理研究所天然产物及糖工程研究组,大连116011

出　　处：《海洋渔业》2016年第1期51-56,共6页Marine Fisheries

基　　金：国家海洋公益性行业科研专项"新型海水养殖疾病防治绿色系列产品研发及其产业化应用"(项目编号201405003);国家科技支撑项目"大型海藻新型产品加工技术"(项目编号2013BAB01B01);大连市科技兴海专项"安全健康刺参育苗新模式构建"(项目编号20140101);中科院青年创新促进会(项目编号2015144)

摘　　要：研究了褐藻酸钠寡糖(AOS)和壳寡糖(COS)对仿刺参(Apostichopus japonicus)生长及免疫功能的影响。实验分为对照组、COS组和AOS组,对照组投喂企业常规饲料,COS组在对照组饲料基础上添加壳寡糖(1 g·kg^(-1)),AOS组在对照组饲料基础上添加褐藻酸钠寡糖(1 g·kg^(-1))。在第30、60、90天,测定各组仿刺参的质量,并在第90天随机采集50 ind仿刺参的体腔液,测定酸性磷酸酶、碱性磷酸酶、过氧化氢酶和一氧化氮合酶等免疫酶的活力。结果表明,AOS组和COS组仿刺参体质量分别比对照组增长16.4%和18.2%;AOS组仿刺参的酸性、碱性磷酸酶活性分别提高了75.1%和67.3%;而COS组仿刺参的过氧化氢酶和一氧化氮合酶的活性分别提高了190.3%和55.6%。海洋寡糖COS和AOS作为仿刺参的免疫增强剂,可有效提高幼参的生长性能和免疫力,其中COS的作用效果优于AOS。Apostichopus japonicus is a valuable species with nutritional and medicinal value in Holothuroidea. In recent years, sea cucumber business in North China has been growing fast. The production of sea cucumber in North China is about 40% of the total domestic production, and the output value is more than ten billion RMB. However, the frequent occurrence of sea cucumber disease leads to the abuse of antibiotics. It is significant to decrease the use of antibiotics. The functional oligosaccharides, as a new immunopotentiator, have been applied in the breeding of livestock and poultry. And its application in the breeding of marine lives has been a hot spot in recent years. The paper investigated the influence of alginate oligosaccharides and chitosan oligosaccharides during sea cucumber breeding to increase the production, improve the disease resistance of sea cucumbers effectively and to reduce the amount of antibiotic during the breeding process. The test cycle was 90 days, and three groups, control check, alginate oligosaccharides and chitosan oligosaccharides were investigated. Control check was fed according to the average feed standard. Chitosan oligosaccharide and alginate oligosaccharide groups were fed with chitosan oligosaccharides and alginate oligosaccharides （1 g · kg-1） respectively in addition to the average feed standard. The juvenile sea cucumber （0. 425 kg per pond） was fed in 20 m3 pond respectively. Each group was weighed after 30 days, 60 days and 90 days and the coelomic fluid of 50 randomly selected sea cucumbers in each group were gathered after 90 days and their immune enzyme activities, acid phosphatase, alkaline phosphatase, catalase, nitricoxide synthase were tested. Results showed that alginate oligosaccharide and chitosan oligosaccharide increased the sea cucumber growth rate by 16. 4% and 18. 2% respectively. Alginate oligosaccharide increased the acid phosphatase activity by 75. 1% and the alkaline phosphatase activity by 67.3%. The chitosan oligosaccharide increased the catala

关 键 词：仿刺参 免疫增强剂 壳寡糖 褐藻酸钠寡糖 

分 类 号：S963.3[农业科学—水产养殖]