翠云草总生物碱对小鼠S180实体瘤的抑制作用  

Inhibition Effect of Total Alkaloid of Selaginella uncinata(Desv.)Spring on S180 Solid Tumor in Mice

作　　者：苏恒海 孙会静 李丽[1] 周静[1] 

机构地区：[1]广西中医药大学,广西南宁530222 [2]广西壮族自治区人民医院,广西南宁530021

出　　处：《广西科学》2015年第6期646-650,共5页Guangxi Sciences

基　　金：广西自然科学基金项目(2012GXNSFAA053147);广西中药质量标准研究重点实验室开放课题基金项目(桂中重开201105);广西"百人计划"项目(J13168)资助

摘　　要：【目的】研究翠云草Selaginella uncinata(Desv.)Spring总生物碱(TAS)对小鼠腋下S180实体瘤的抑制作用及抗肿瘤机制。【方法】分析空白对照组、5-FU阳性药对照组、TAS大和中剂量(300 mg·kg^(-1)、150 mg·kg^(-1))组S180实体瘤重抑制率、NF-κB及COX-2的表达量,并观察各组切片。【结果】TAS对S180实体瘤具有抑制作用。TAS大、中剂量(300mg·kg^(-1)、150mg·kg^(-1))组的瘤重、NF-κB和COX-2的表达量均显著低于空白对照组(P<0.05或P<0.01)。【结论】TAS能明显抑制S180实体瘤的生长,其抗肿瘤机制可能是通过抑制NF-κB和COX-2的表达,继而抑制向肿瘤输送养份和氧气的血管的生长,从而实现抗肿瘤作用。【Objective】The total alkaloid of Selaginella uncinata(Desv.)Spring(TAS)was extracted,and then its inhibition effect on S180 solid tumor in mice and the antitumor mechanism were studied.【Methods】The weight inhibition rate against S180 solid tumor,the expression of NF-κB and COX-2were analyzed in the blank control group,5-FU positive control group,and TAS large and middle dose groups(300mg·kg^(-1) and 150mg·kg^(-1) body weight),combined with observation of the sections from different groups.【Results】All the tumor weigh and the NF-κB/COX-2concentration in the TAS high-and mid-dose groups(300mg·kg^(-1) and 150mg·kg^(-1))were statistically less than those in the control group(P <0.05 or P <0.01).【Conclusion】TAS can curb or suppress tumor growth.Its antitumor mechanism is probably to inhibit the expression of NF-κB/COX-2,thereby to inhibit the growth of the blood vessel around the tumor,by which tumor growth is curbed or suppressed.

关 键 词：翠云草 总生物碱 抗肿瘤 作用机制 

分 类 号：R285.5[医药卫生—中药学]