硫酸庆大霉素中RNA和DNA的检查  

Determination of RNA and DNA in Gentamicin Sulfate

作　　者：杨帆[1,2] 夏云空 刘晓薇 席德慧[1] 

机构地区：[1]四川大学生命科学学院,四川成都610042 [2]成都蓉生药业有限责任公司,四川成都61000

出　　处：《生物技术世界》2016年第2期166-167,共2页Biotech World

摘　　要：目的 为检查硫酸庆大霉素中的RNA与DNA,方法 先用凝胶柱色谱法分离出RNA与DNA,再采用改良苔黑酚法检查RNA,采用二苯胺法检查DNA.结果 RNA在1-20μg范围内,吸光度与浓度线性回归的相关系数r=0.9993,检测限和定量限分别为0.28μg和0.85μg,平均回收率为96.1%;DNA在5-200μg范围内,吸光度与浓度线性回归的相关系数r=0.9995,检测限和定量限分别为2.8μg和6.3μg,平均回收率为93.3%.结论 所建立的方法专属、灵敏、准确,可用于硫酸庆大霉素中RNA 和DNA的检查.Objective: To determine RNA and DNA in Gentamicin Sulfate, Methods: RNA and DNA were separated by gel chromatography and determined with improved orcinol colorimetry and diphenylamine colorimetry, respectively. Results: For RNA determination, the linear range was 1~20 μg(r=0.9993). LOD and LOQ were 0.28μg 0.85μg, respectively. The average recovery was 96.1%. For DNA determination, the linear range was 5~200 μg(r=0.9995). LOD and LOQ were 2.8μg 6.3μg, respectively. The average recovery was 93.3%. Conclusion: The methods were specific, sensitive, accurate and suitable for determination of RNA and DNA in Gentamicin Sulfate.

关 键 词：硫酸庆大霉素 RNA DNA 凝胶柱色谱法 改良苔黑酚法 二苯胺法 

分 类 号：R917[医药卫生—药物分析学]