泉水鱼的细胞遗传学分析  

Cytogenetic Analysis of Pseudogyrinocheilus prochilus

作　　者：邹远超[1] 文正勇 岳兴建[1] 王永明[1] 覃川杰[1] 陶敏[1] 李斌[1] 谢碧文[1] 王淯[1] 齐泽民[1] 

机构地区：[1]内江师范学院生命科学学院,长江上游鱼类资源保护与利用四川省重点实验室,内江641112

出　　处：《动物学杂志》2016年第1期66-72,共7页Chinese Journal of Zoology

基　　金：四川省教育厅科技创新科研团队基金项目(No.KYTD201009);四川省教育厅项目(No.15ZA0285)

摘　　要：以泉水鱼(Pseudogyrinocheilus prochilus)的肾为材料,采用体内注射植物血球凝集素(PHA)和秋水仙素、肾组织细胞短期培养、常规空气干燥法制备泉水鱼染色体标本,对其核型进行分析。以泉水鱼外周血细胞为样本,鸡(Gallus gallus)血细胞DNA含量(2.50 pg/2c,2c指二倍体)为标准,用流式细胞仪测定了泉水鱼的DNA含量。结果表明:(1)泉水鱼的染色体数量为2n=50,核型公式为12m+14sm+14st+10t,总臂数NF=76,未发现性别相关的异型染色体;(2)泉水鱼的DNA含量为鸡血对照的(1.05±0.04)倍,其绝对DNA含量为(2.62±0.10)pg/2c。泉水鱼的染色体数目和DNA含量显示出二倍体的特征。The chromosome number and karyotype of Pseudogyrinocheilus prochilus were analyzed using a routine method including intraperitoneal injection of phyohemagglutinin（PHA） and colchicine, cultivation invivo, and slides preparation with air-drying. Erythrocyte nuclear DNA content of P. prochilus was determined by using a flow cytometer（Beckman Coulter Inc., USA） with the chicken（Gallus gallus） erythrocytes（their DNA content is 2.50 pg/2c） serving as reference cells. The results showed that the diploid chromosome number of P. prochilus was 2n = 50, and the karyotype of this species was 2n = 12 m ＋ 14 sm ＋ 14 st ＋ 10 t, NF = 76（Fig. 1 and Table 1）. The heterosomes were not observed. The diploid cellular DNA content of P. prochilus was 2.62 ± 0.10 pg/2c（n = 20）, which was 1.05 ± 0.04 times of that of the chicken erythrocytes（Fig. 2）. The result of karyotype and DNA contents of P. prochilus indicate that P. prochilus is diploid.

关 键 词：泉水鱼 染色体 核型 DNA含量 

分 类 号：S917.4[农业科学—水产科学]