AMPK腺病毒载体在小鼠骨骼肌中的表达和作用  

Effect and expression of AMPK adenovirus on mouse skeletal muscle

作　　者：刘倩[1] 胡芳[1] 牛文彦[1] 

机构地区：[1]天津医科大学免疫学系,天津300070

出　　处：《天津医科大学学报》2016年第1期1-4,共4页Journal of Tianjin Medical University

基　　金：国家自然科学基金面上项目基金资助(81170740);高等学校博士学科点专项基金(20121202110014);天津市科委应用基础研究重点项目(15JCZDJC35500)

摘　　要：目的:探讨注射AMPK腺病毒在小鼠骨骼肌中的表达和作用。方法:C57BL/6小鼠随机分为4组:(1)无任何处理的空白对照组。(2)肌肉注射腺病毒空载体(Ad-GFP)组。(3)肌肉注射Ad-GFP,48 h后腹腔注射AMPK激活剂AICAR组。(4)肌肉注射GFP标记的激活型AMPK腺病毒(Ad-AMPK-CA)组。注射腺病毒72 h后,处死小鼠,通过小动物成像系统测定骨骼肌中的荧光强度,检测腺病毒的表达,用Western blot方法检测ACC的磷酸化。结果:与空白对照组比较,注射腺病毒组的平均荧光强度显著增高。与病毒空载体组比较,注射AICAR组和Ad-AMPK-CA组的ACC磷酸化水平显著升高。结论:AMPK腺病毒能在小鼠骨骼肌中表达目的蛋白,调节AMPK的作用。Objective： To explore the effect and expression of injected AMPK adenovirus on mouse skeletal muscle. Methods： Ten weeks old C57BL/6 male mice were randomly divided into four groups. Mice in groupl were normal control group without treatment. Mice in group 2 were intramuscularly injected green fluorescence protein adenovirus （Ad-GFP）. Mice in group 3 were intramuscularly injected Ad-GFP, after which were intraperitoneally injected AMPK activator AICAR in 48 h. Mice in group 4 were intramuscularly injected Ad- AMPK-CA. Seventy two hours after adenovirus treatment, all mice were executed and the expression of adenovirus was analysed in skeletal muscle. Fluorescence intensity and ACC phosphorylation in skeletal muscle were analysed by vivo imaging system and western blot, respectively. Results： Compared with the control group, the mean fluorescence intensity of the experimental groups which were injected adenovirus was significantly higher. Compared with the Ad-GFP group, ACC phosphorylation of AICAR and Ad-AMPK-CA groups were significantly increased. Conclusion： AMPK adenovirus can express the target protein in mouse skeletal muscle, and regulate the activity of AMPK.

关 键 词：AMPK 腺病毒 小鼠 骨骼肌 

分 类 号：R392.1[医药卫生—免疫学]