青蒿素类药联合紫杉醇对小鼠黑色素瘤B16细胞的增殖抑制作用  

Antitumor Effect of Artemisinins Combined with Paclitaxel on B16 Mouse Melanoma Cells

作　　者：薛林路 李国铭[2] 邓长生[1] 王琪[1] 徐勤[1] 宋健平[1,2] 

机构地区：[1]广州中医药大学,广东广州510006 [2]广州中医药大学科技产业园,广东广州510445

出　　处：《中药新药与临床药理》2016年第1期49-53,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(81273643);青年科学基金项目(81403295)

摘　　要：目的观察青蒿琥酯(ATS)、双氢青蒿素(DHA)和紫杉醇(Taxol)及ATS联合Taxol对小鼠黑色素瘤B16细胞的增殖抑制作用,评价各组药物对小鼠黑色素瘤B16细胞的杀伤作用,初步探讨ATS与Taxol联合给药对小鼠黑色素瘤B16细胞是否有协同增效作用。方法采用CCK-8法检测ATS、DHA和Taxol单药对小鼠黑色素瘤B16细胞作用24 h和48 h的抑制作用,并计算半数抑制浓度(IC_(50))值;检测ATS联合Taxol不同配比AT(1∶1)、AT(3∶2)和AT(7∶3)对小鼠黑色素瘤B16细胞作用48 h的抑制作用。运用质量作用中效定理和药物合并指数定理分析两药联合是否具有协同增效作用。结果 ATS、DHA和Taxol单药作用于小鼠黑色素瘤B16细胞的IC_(50)值,在24 h时,分别为126.56,86.09,3.27μmol·L^(-1);在48 h时,分别为1.36,15.11,0.97μmol·L^(-1)。ATS联合Taxol 3个浓度组AT(1∶1)、AT(3∶2)和AT(7∶3)对小鼠黑色素瘤B16细胞作用48 h,联合用药合并指数(CI)均小于1。结论 ATS、DHA和Taxol单药对小鼠黑色素瘤B16细胞的增殖抑制作用随着药物浓度和时间的增加而明显增加,呈剂量和时间依赖性。青蒿琥酯联合紫杉醇抑制小鼠黑色素瘤B16细胞增殖,有良好的协同增效作用。Objective To observe the inhibitory effects of artesunate（ATS）, dihydroartemisinine（DHA）and paclitaxel（Taxol）alone,and ATS combined with Taxol on the proliferation of B16 mouse melanoma cells,so as to evaluate the killing effects of each treatment on B16 mouse melanoma cells and to preliminarily explore the synergistic action of ATS combined with Taxol on B16 mouse melanoma cells. Methods CCK-8 was used for observing the inhibitory effects of ATS,DHA and Taxol alone on B16 mouse melanoma cells after co-culturing for over 24 h and 48 h,and then the 50 % inhibition concentration（IC_（50）） was calculated. The inhibitory effects of ATS combined with Taxol at different ratios（1 ∶1, 3 ∶2 and 7 ∶3） on B16 mouse melanoma cells were detected after co-culturing for over 48 h.Median-effect theory and drug combination index were used to analyze the synergistic action of the combination of ATS-Taxol. Results The IC_（50） of ATS, DHA and Taxol alone on B16 mouse melanoma cells at hour 24 was 126.56,86.09 and 3.27 μmol·L^（-1）,respectively,and 1.36,15.11,0.97 μmol·L^（-1） at hour 48,respectively. The drug combination index（CI）was 1 for ATS-Taxol at all combination ratios of 1∶1,3∶2 and 7∶3 after co-culturing with B16 mouse melanoma cells over 48 h. Conclusion ATS,DHA and Taxol alone exerts inhibitory effects on B16 mouse melanoma cells in dose- and time-dependent manner,and ATS combined with Taxol has favorable synergistic action on inhibiting the proliferation of B16 mouse melanoma cells.

关 键 词：青蒿琥酯 紫杉醇 小鼠黑色素瘤B16细胞 

分 类 号：R285.5[医药卫生—中药学]