β-细辛醚对阿尔茨海默病细胞模型的作用及对神经元突触功能可塑性的影响  

Effect of β-asarone on In-vitro Alzheimer's Disease(AD) Cell Model and Its Influence on Neuronal Synaptic Plasticity

作　　者：刘四军[1] 陈惠芳[2] 张一帆[2] 王莹莹[2] 陈云波[2] 王奇[2] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学临床药理研究所,广东广州510006

出　　处：《中药新药与临床药理》2016年第1期54-57,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广州中医药大学优秀青年学者科研基金(201303);广东省自然科学基金项目(2015A030310414);教育部高等学校博士学科点专项科研基金(20114425110007)

摘　　要：目的探索β-细辛醚对于阿尔茨海默病(Alzheimer's Disease,AD)细胞模型的作用及其可能机制。方法选用Aβ1-42的浓度为10μmol·L^(-1)作为建模条件,以NG108-15细胞为研究对象,建立体外AD细胞模型,β-细辛醚低、中、高剂量组(6.25,12.5,25μmol·L^(-1))分别进行干预,通过MTT法检测细胞活力,并观察β-细辛醚对细胞形态学的影响以及Wesstern blot法检测细胞突触的相关功能蛋白突触素蛋白(Synaptophysin,SYP)、以及AMPA受体亚单位(Glutamatergic receptor 2,GluR2)的表达。结果 MTT结果显示,β-细辛醚高剂量组干预24 h后能显著减轻Aβ1-42的毒性损伤(P<0.05)。从细胞形态上看,细辛醚各剂量组跟模型组差异显著,跟溶媒对照组比较无明显差异。与模型组SYP相对表达量比较,溶媒对照组、β-细辛醚中、高剂量组差异均有统计学意义(P<0.05)。β-细辛醚中、高剂量组的SYP表达量显著上调。而各组GluR2相对表达量比较,差异均无统计学意义(P>0.05)。结论β-细辛醚高剂量组能显著改善细胞形态以及提高细胞活力,具有一定的神经保护作用,可能是通过上调SYP的表达,从而影响突触功能,改善认知。Objective To explore the effect and the possible mechanism of β-asarone on Alzheimer＇s disease（AD）in-vitro cell model. Methods Selecting 10 μmol·L^（-1）A β1-42 as modeling conditions and using NG108-15 cells as the research object,we established the AD cell model in vitro. After the cell model was treated with different doses ofβ-asarone（6.25,12.5,25 μmol·L^（-1））,we detected the cell viability by MTT method,and investigated the effects ofβ-asarone on cell morphology and on the expression of synaptophysin（SYP）and glutamatergic receptor 2（Glu R2）.Results The MTT assay results showed that 25 μmol·L^（-1） of treatment with high dose of β-asarone for 24 h significantly reduced the toxicity of Aβ1-42（P〈0.05）. The results of morphological examination showed that the difference of cell morphology was significant between the three β-asarone groups and model group, but was insignificant betweenβ-asarone groups and the normal group. Compared with the model group,SYP relative expression in medium- and high-dose β-asarone groups was up-regulated（P〈0.05）. However,the difference of Glu R2 relative expression was not significant between any two groups（P〈0.05）. Conclusion β-asarone at 25 μmol/L could significantly improve the cell morphology and improve the cell vitality,and its neuroprotective mechanism is possibly related to upregulating the expression of SYP,thereby affecting synaptic function and improving cognition.

关 键 词：Β-细辛醚 阿尔茨海默病 神经元 突触功能可塑性 

分 类 号：R285.5[医药卫生—中药学]