葛根素抑制大鼠膀胱逼尿肌舒缩活动实验  

Inhibitory effect of puerarin on activity of isolated bladder detrusor muscle in rats

作　　者：龙湟哲 姜华茂[1] 田洪阳[1] 王海光[1] 贾凡振 左知润 

机构地区：[1]辽宁医学院泌尿外科,辽宁锦州121000

出　　处：《解放军医学院学报》2016年第1期79-82,共4页Academic Journal of Chinese PLA Medical School

基　　金：辽宁省科学技术计划项目资助项目(2013225305);辽宁医学院校长基金资助项目(AH2014013)~~

摘　　要：目的探讨葛根素对大鼠逼尿肌舒缩活动的影响及其作用机制。方法 56只SD雌性大鼠于T10处横断脊髓建立骶上脊髓损伤大鼠模型,随机分为葛根素10 mg组、葛根素20 mg组、葛根素30 mg组、对照组,每组14只,按组别分别腹腔注射葛根素注射液10 mg、20 mg、30 mg及等体积0.9%氯化钠注射液,1次/d,连续1个月后,行尿动力学检测,比较各组膀胱最大容量(maximum bladder capacity,MBC)、漏尿点压(bladder leak point pressures,BLPP)及膀胱收缩频率(contraction frequency,CF),Western blot检测葛根素组膀胱T型钙离子通道亚型(α1G)及酪氨酸激酶受体(C-kit)表达情况。结果尿动力学显示葛根素组MBC[(3.375 0±0.074 7)ml]较对照组[(0.804 9±0.114 1)ml]增大,BLPP[(23.82±0.31)cm H2O(1 cm H2O=0.098 k Pa)]和CF[(5.24±0.12)次/s]均较对照组BLPP[(29.62±1.82)cm H_2O]、CF[(8.72±0.38)次/s]下降(P<0.01);葛根素组内MBC 30 mg组[(3.982 6±0.162 7)ml]>20 mg组(3.261 9±0.135 2)ml和10 mg组(2.836 7±0.216 5)ml,BLPP10 mg组[(25.08±1.71)cm H_2O]、20 mg组[(23.78±1.37)cm H_2O]和30 mg组[(22.68±1.91)cm H_2O]依次降低,CF在10 mg组、20 mg组、30 mg组依次降低,分别为(6.13±0.15)次/s、(5.21±0.22)次/s、(4.34±0.12)次/s(P<0.01);Western blot示葛根素10 mg组、20 mg组、30 mg组α1G表达量依次下降,分别为(0.832±0.065)、(0.541±0.051)、(0.324±0.059),C-kit表达量在葛根素10 mg组、20 mg组、30 mg组中分别为(0.673±0.059)、(0.519±0.019)、(0.316±0.038)(P<0.01)。结论葛根素可抑制膀胱逼尿肌的收缩活动,其机制可能与膀胱逼尿肌内C-kit和α1G下调有关。Objective To investigate the effects of puerarin on the activity of bladder detrusor muscle in rats and explore its mechanism. Methods Complete spinal cord transaction was established by cutting out of dura at T10 in 56 female SD rats and rats were randomly allocated into puerarin 10 mg, puerarin 20 mg, puerarin 30 mg and control groups. Each group was intraperitoneally injected with puerarin（10 mg, 20 mg, 30 mg） and 0.9% sodium chloride, respectively, once a day for 30 days. Then Urodynamic monitor was performed to evaluate maximum bladder capacity（MBC）, bladder leak point pressures（BLPP） and contraction frequency（CF） in rats of each group, western blot was used to measure the expression of subtype of T-type calcium channelα1G and C-kit under interventions of puerarin in each group. Results Urodynamic showed that MBC in puerarin group was（3.375 0±0.074 7） ml and（0.804 9±0.114 1） ml in saline group, BLPP and CF in puerarin group were（23.82±0.31） cm H2O and（5.24±0.12）/s respectively, which were significantly lower than saline group [（29.62±1.82） cm H2O,（8.72±0.38）/s, P〈0.01]. MBC in puerarin 30 mg, puerarin 20 mg, puerarin 10 mg groups were（3.982 6±0.162 7） ml,（3.261 9±0.135 2） ml and（2.836 7±0.216 5）ml respectively, BLPP in puerarin 10 mg, puerarin 20 mg, puerarin 30 mg groups were（25.08±1.71） cm H2O,（23.78±1.37） cm H2O and（22.68±1.91） cm H2O, which showed successively decline, CF in puerarin 10 mg, puerarin 20 mg, puerarin 30 mg groups were（6.13±0.15）/s,（5.21±0.22）/s,（4.34±0.12）/s, which showed significant decrease（P〈0.01）. Western blot showed that the expression quantity of α1G in puerarin（10, 20, 30 mg） groups were（0.832±0.065）,（0.541±0.051） and（0.324±0.059）, and the expression quantity of C-kit in puerarin（10, 20, 30 mg） groups were（0.673±0.059）,（0.519±0.019）,（0.316±0.038） with significant decrease（P〈0.01）. Conclusion Puerarin can inhibit the contraction of isolated

关 键 词：葛根素 T型钙通道 膀胱起搏细胞 

分 类 号：R694[医药卫生—泌尿科学]