HPLC法测定养阴清肺丸中芍药苷的含量  

DETERMINATION OF THE PAEONIFLORIN IN YANGYINQINGFEI PILLS BY HPLC

作　　者：许倩云[1] 王玉华[2] 

机构地区：[1]内蒙古医科大学附属医院耳鼻喉科,内蒙古呼和浩特010050 [2]内蒙古医科大学

出　　处：《内蒙古医科大学学报》2015年第4期314-317,共4页Journal of Inner Mongolia Medical University

基　　金：内蒙古自治区科技创新引导奖励资金项目(20121502)

摘　　要：目的：建立养阴清肺丸中芍药苷的定量方法。方法：高效液相色谱法,色谱柱为Phenomen C18柱（250 mm×4.6 mm,5μm）,流动相为甲醇-0.05mol/L磷酸二氢钾溶液（40∶65）,检测波长为230nm。结果：芍药苷测定的线性范围是0.108~1.00μg（r=0.9999）;平均回收率为99.72%,RSD为1.18%（n=5）。结论：该方法简便、灵敏、准确,重现性好,可用于养阴清肺丸中芍药苷的含量测定。Objective： To determine the content of Paeoniflorin in Yangyinqingfei pills. Methods：The content of Paeoniflorin in Yangyinqingfei pills was determined by HPLC with Diamonsil-C18（ 250nm×4. 6mm,5μm） column. The mobile phase was methanol-0. 05mol/L Potassium dihydrogen phosphate solution（ 40 ∶65）. The wavelength was set at 230 nm. Results： The linear range was 0. 108 ~1. 00μg / m L and correlation coefficient was 0. 9999. The average recovery and the relative standard deviation were 99. 7% and 1. 18%（ n = 5） respectively. Conclusion： The method is simple,accurate and reliable,which can be applied to the determination of Paeoniflorin in Yangyinqingfei pills.

关 键 词：养阴清肺丸 芍药苷 高效液相色谱法 

分 类 号：R289.5[医药卫生—方剂学]