柯萨奇病毒A16型衣壳蛋白VP1～VP3的B淋巴细胞线性抗原表位预测与确定  

Prediction and identification of immunodominant B cell linear epitopes in capsid proteins VP1 to VP3 of Coxsackievirus A16

作　　者：高孟[1] 倪红霞[2] 朱莲[1] 李剑波[1] 高丽美[1] 罗永能[1] 

机构地区：[1]浙江省医学科学院病毒病研究所,杭州310013 [2]宁波市疾病预防控制中心病毒研究所

出　　处：《中华临床感染病杂志》2015年第6期543-548,共6页Chinese Journal of Clinical Infectious Diseases

基　　金：浙江省医学科学院青年基金计划项目（2013Y006）;浙江省医学生物工程疫苗研发重点实验室（2008F3022）

摘　　要：目的 确定柯萨奇病毒A16型（CVA16）YY157株衣壳蛋白VP1～VP3的优势B淋巴细胞线性抗原表位。方法用生物信息学软件Lasergene分析CVA16YY157株衣壳蛋白VP1-VP3氨基酸序列的亲水性、抗原性和表面可及性，预测出包含潜在B淋巴细胞线性抗原表位的区段，并用BepiPred1．0Server进行评估。人工合成相应的多肽片段，用多肽-酶联免疫吸附试验（ELISA）检测其与明确感染CVA16的患儿血清的阳性反应与否和程度，并以健康人血清作比较。结果根据抗原性强、亲水性强和表面性好的要求，共预测出21个可能的B淋巴细胞线性抗原表位。相应的人工合成多肽与感染CVA16的患儿血清均呈现阳性反应，但程度不同。结论成功预测并用实验证实了CVA16YY157株衣壳蛋白VP1～VP3的优势线性B淋巴细胞线性抗原表位。Objective To identify immunodominant B linear cell epitopes in capsid proteins VPI to VP3 of Coxsackievirus A16 （CVA16） strain YY157. Methods The protean algorithms of bioinformatic software Lasergene were used to analyze antigenicity, hydrophilicity and surface probability of amino acid sequence of CVA16 capsid proteins VP1 to VP3. Multiple regions containing potential lineal B cell epitopes were predicted and their corresponding average indexes were calculated by BepiPred 1. 0 Server. Corresponding peptides were synthesized and examined in peptide-enzyme-linked immunosorbent assay （ELISA） individually to check whether it reacted positively or negatively toward sera from children with confirmed CVA16 infection. Results Totally 21 possible B cell linear epitopes were predicted according to their relatively strong antigenicity, hydrophilicity and surface probability. The corresponding synthetic peptides reacted positively with sera of CVA16-infected children in a varying extent. Conclusion Immunodominant B cell linear epitopes of capsid proteins VP1 to VP3 of CVA16 strain YY157 are successfully predicted and confirmed.

关 键 词：柯萨奇病毒感染 表位 B淋巴细胞 酶联免疫吸附测定 

分 类 号：R72[医药卫生—儿科] R4[医药卫生—临床医学]