一株卓贝尔氏菌F13-1好氧反硝化特性及其反硝化基因的初步研究  

Preliminary study on aerobic denitrification characteristics of Zobellella sp.F13-1

作　　者：李小义[1,2] 王丽萍[2,3] 赖其良[2] 李登峰[1] 邵宗泽[1,2,3] 

机构地区：[1]宁波大学海洋学院,浙江宁波31500 [2]国家海洋局第三海洋研究所国家海洋局海洋生物遗传资源重点实验室福建省海洋生物遗传资源重点实验室福建省海洋生物资源开发利用协同创新中心,福建厦门361005 [3]哈尔滨工业大学市政环境工程学院,黑龙江哈尔滨150000

出　　处：《应用海洋学学报》2016年第1期122-129,共8页Journal of Applied Oceanography

基　　金：厦门南方海洋研究中心资助项目(14CZP034HJ08);海洋公益性行业科研专项资助项目(2010005032);国家微生物资源平台资助项目(NIMR-2014-9)

摘　　要：为获得高效好氧反硝化细菌,并研究这些细菌的脱氮特性以及好氧反硝化机理,采集了近海表层沉积物进行富集培养,分离获得一株具有高效好氧反硝化能力的菌株F13-1.结合生理生化特征及16S rRNA基因序列分析,初步鉴定菌株F13-1为卓贝尔氏菌(Zobellella sp.).在以葡萄糖为碳源,C/N比值为15,盐度为30,摇床转速160 r/min,p H值为7,28℃的最优脱氮条件下菌株F13-1脱氮效果较好,分别能将100 mg/dm3硝酸盐和亚硝酸盐在24和16 h内彻底脱除,脱除速率分别为:5.87 mg/(dm3·h)和5.97 mg/(dm3·h).并且菌株F13-1对高浓度亚硝酸盐具有较好的耐受性,能在高浓度亚硝酸盐存在条件下正常生长及脱氮,156 h内能将400 mg/dm3亚硝酸盐脱除完全.基因组分析表明,该菌株具有完整的反硝化基因簇,包括nap、nir、nor和nos等共17个基因.研究结果表明该菌株具有高效好氧反硝化特性,在养殖废水处理中具有较好的应用潜力.An aerobic denitrifying bacterium,designated strain F13-1,was isolated from marine sediments collected from Houlong Dock,Quanzhou. Further the phylogenetic and denitrifying characteristic was investigated. Based on its biochemical properties and the results of 16 S rRNA gene analysis,strain F13-1 was identified as Zobellella sp..Under the optimal conditions of glucose as carbon source,C / N ratio at 15,salinity of 30,p H 7,28℃,with shaking speed 160 r / min,strain F13-1 exhibited highest nitrogen removal efficiency,which could reduce nitrate and nitrite nitrogen rapidly from 100 mg / dm3 to 0 mg / dm3 within 24 and 16 h,respectively. The denitrification rate was5. 87 mg /（ dm^3·h） and 5. 97 mg /（ dm^3·h）,respectively. The NO-2-N of 400 mg / dm3 can be completely removed by strain F13-1 at the optimal growth conditions. Four gene clusters associated with denitrification pathway：nap,nir,nor and nos,including 17 genes in total,were identified in the genome of strain F13-1. This study provides an aerobic denitrifying bacterium with high nitrogen removal efficiency with potential application in marine aquaculture and an useful material for studying aerobic denitrifying mechanisms.

关 键 词：海洋生物学 生物脱氮 好氧反硝化 卓贝尔氏菌 反硝化基因 

分 类 号：P735[天文地球—海洋生物学]