基于COⅠ基因的厦门海域鱼类DNA条形码鉴定  

Species identification for fishes in Xiamen sea waters based on COⅠbarcoding

作　　者：邢炳鹏[1] 林汝榕[1] 王彦国[1] 张稚兰[1] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005

出　　处：《应用海洋学学报》2016年第1期144-150,共7页Journal of Applied Oceanography

基　　金：福建省科技厅计划资助项目(2013N0021);国家海洋局第三海洋研究所基本科研经费资助项目(海三科2013001)

摘　　要：为研究DNA条形码技术在厦门海域鱼类分类鉴定中的可行性,随机选取了厦门海域23种鱼类样品进行COⅠ基因扩增,结果共获取23条序列,平均长度为656 bp,序列中T、C、G、A碱基的平均含量分别为:29.40%、28.10%、18.40%、24.10%.样品种内、种间、科内和目内的遗传距离(K2P)分别为:0.21%,20.50%、24.47%和25.62%,遗传距离随着分类阶元的提高而增大,种间遗传距离明显大于种内遗传距离.所选厦门海域鱼类样品仅蓝圆鯵鉴定到圆鯵属,未能鉴定到种,其余22种全部鉴定到种,表明COⅠ基因序列可以作为鱼类分类鉴定的有效DNA条形码.In this study,we collected DNA barcodes based on cytochrome oxidase subunit Ⅰ（ COⅠ） sequences from23 species of fish specimens captured in Xiamen sea waters. The mean length of the sequences was 656 bp and theaverage content of T,C,G and A were 29. 4%,28. 1%,18. 4% and 24. 1%,respectively. The average Kimura two parameter（ K2P） distances within-species,genus,family and order were 0. 21%,20. 50%,24. 47% and25. 62%,respectively. Interspecific genetic distance was much larger than intraspecific distance from composition.K2 P genetic distance values were found increasing with taxonomic level. All species,except for Decapterus maruadsi,can be differentiated by their COⅠ sequence. High efficiency of species identification was demonstrated in the present study by DNA barcoding,and we conclude that COⅠ sequencing can be used to identify fish species.

关 键 词：海洋生物学 鱼类 细胞色素C氧化酶亚基Ⅰ基因 DNA条形码 物种鉴定 厦门海域 

分 类 号：P735[天文地球—海洋生物学]