知母皂苷对Aβ_(1-42)诱导的皮层神经元突触损伤的保护作用  

Protective effect of Saponin B on Aβ_(1-42) induced synaptic damage in cultured cortical neurons

作　　者：隋海娟[1] 马瑞国 刘佳颖[3] 刘卓[1] 闫恩志[1] 金英[1] 

机构地区：[1]辽宁医学院药理学教研室 [2]锦州奥鸿药业有限责任公司 [3]辽宁医学院医疗学院,锦州121001

出　　处：《中药药理与临床》2015年第6期27-30,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：辽宁省科学技术基金项目(2013022008)

摘　　要：目的:探讨知母皂苷(SAa B)对Aβ_(1-42)诱导的皮层神经元损伤是否有保护作用。方法:MTT法测皮层神经元细胞存活率;免疫荧光染色法检测神经元突触素(SYP)、突触后致密蛋白95(PSD-95)表达的改变。Western印迹法检测神经元SYP、PSD-95、p-Akt473、p-m TOR蛋白表达水平表达的改变。结果:Aβ_(1-42)作用48 h可使大鼠大脑皮层神经元细胞存活率明显降低,使神经元SYP、PSD-95、p-Akt473和p-m TOR蛋白表达明显降低。SAa B(0.01,0.1,1,5,10μmol/L)可明显对抗Aβ_(1-42)引起的神经元细胞存活率下降,并呈一定浓度依赖性。SAa B也可使SYP、PSD-95、p-Akt473和p-m TOR蛋白表达明显增加。结论:知母皂苷能够抑制Aβ_(1-42)诱导的皮层神经元突触损伤,这种作用可能与PI3K/Akt/m TOR信号转导通路有关。Objective：To investigate whether saponin B can protect cortical neurons from Aβ1-42 induced synaptic damage. Methods： The cell via- bility was assessed by MTT assay. Immunstaining were used for SYP and PSD-95 protein expression. The protein expression of SYP, PSD- 95, p-Akt473 and p-mTOR were determined by Western blotting. Results： The decrease of cell viability and the decrease of SYP, PSD-95, p-Akt473 and p-mTOR protein in cultured cortical neurons were observed incubated with A13142 for 48 h compared with the normal controls. SAaB （0.01, 0.1, 1, 5, 10 βmoL/L） inhibited Aβ1-42-induced decrease in cell viability, and showed a dose-dependent. SAaB induced the increases of SYP, PSD-95, p-Akt473 and p-roTOR protein expression. Conclusion：SAaB can inhibited the Aβ1-42-induced synaptic damage in cultured cortical neurons, which mainly via activated P13K/Akt/mTOR signal pathway.

关 键 词：知母皂苷 阿尔茨海默病 Β淀粉样蛋白 皮层神经元 突触素 突触后致密蛋白95 

分 类 号：R285[医药卫生—中药学]