黄芪甲苷对H9c2心肌细胞能量代谢的影响及其机制  

The effect and mechanism of astragaloside IV on energy metabolism of H9c2 cardiomyocytes

作　　者：许放[1] 郭斌[2] 韩冠英[1] 张素萍[3] 王洪新[3] 

机构地区：[1]辽宁医学院药学院,锦州121000 [2]辽宁医学院附属第一医院,锦州121000 [3]辽宁医学院心脑血管药物重点实验室,锦州121001

出　　处：《中药药理与临床》2015年第6期36-39,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：辽宁医学院校长基金奥鸿博泽基金XZJJ20130106-02

摘　　要：目的:探讨黄芪甲苷(Astragaloside IV,As IV)对H9c2心肌细胞能量代谢的影响及其机制。方法:H9c2心肌细胞培养,传代后随机分为6组,空白组、模型组、不同浓度黄芪甲苷用药组及普萘洛尔组。培养液中分别加入黄芪甲苷(25、50、100μmol/L)及普萘洛尔(2μmol/L)预处理,30min后加入Iso(10μmol/L)继续培养48 h。消化分离法及计算机图像分析系统检测细胞体积;考马斯亮蓝法检测细胞中蛋白的量;real-time RT-PCR法检测心肌细胞心房钠尿肽(ANP)mRNA的表达;Western blotting法检测心肌细胞PGC-1α、PDK4蛋白的表达;酶联免疫吸附测定(Elisa)法检测细胞外液中三磷酸腺苷(ATP)、单磷酸腺苷(AMP)及游离脂肪酸(FFA)的含量。结果:与正常对照组相比,模型组心肌细胞体积明显增大,总蛋白的量增加,ANP mRNA表达上调,PGC-1α、PDK4蛋白表达下调,心肌细胞中FFA含量及ATP/AMP比值减少。黄芪甲苷(50、100μmol/L)、普萘洛尔(2μmol/L)均能有效抑制Iso诱导的心肌细胞肥大,使心肌细胞体积减小,总蛋白的量降低,ANP mRNA表达下调,PGC-1α、PDK4蛋白表达上调。结论:黄芪甲苷对Iso诱导的H9c2心肌细胞肥大有保护作用,改善肥大心肌细胞的能量代谢表达水平,其机制可能与PGC-1α信号通路有关。Objective： To explore the effect of Astragaloside IV on energy metabolism of H9c2 cardiomyocytes and its underlying mechanism. Methods： H9c2 myocardial cells were cultured and randomly divided into six groups： Control group, Model group, pre-treatment Astragalo- side IV groups and Propranolol group. 25,50 and 100μmol/L of Astragaloside IV and propranolol （2μmol/L） was added into cell culture medium for 30mins before 48 h incubation with Iso 10μmol/L. The cardiomyocyte volume was measured by computer photograph analysis sys- tem. Total protein contents were assayed by the method of Bradford. Real-time RT-PCR was used to quantify the mRNA expression of ANP; Western blotting was used to quantify the expression of PGC-1 α, PDK4 proteins; Elisa was used to quantify adenosine triphosphate （ATP）, adenosine, monophosphate （AMP） and free fatty acids （FFA）. Results： Compared with the Control group, the cell size, total protein con- tent, ANP mRNA, FFA level were increased, and the protein expression of PGC-1 α, PDk4 were decreased in Iso group. Astragaloside IV （50,100 μmol/L）, propranolol （2 μmol/L） could remarkably down-regulate the over-expression of the cell size, total protein content, ANP mRNA and increase the protein expression of PGC-1 α, PDk4. Conclusions： Astragaloside IV has the protective effect on H9c2 cardiomyo- cyte induced by Iso and could also ameliorate the energy metabolism in hypertrophic cardiomyocytes, which is partially through the PGC-lα signaling pathway.

关 键 词：黄芪甲苷 H9C2心肌细胞 能量代谢 PGC-1α信号通路 

分 类 号：R285[医药卫生—中药学]