哮喘患儿外周血CD4^+CD25^(nt/hi)CD127^(lo)调节性T细胞的测定及临床意义  

The level of CD4^+CD25^(nt/hi)CD127^(lo) regulatory T cells and its clinical significance in children with asthma

作　　者：湛洁谊[1] 卢慧敏[1] 林穗玲 

机构地区：[1]广州医科大学附属第一医院儿科,广东广州510120 [2]广州天河区妇幼保健院检验科,广东广州510610

出　　处：《中国实验诊断学》2016年第1期78-80,共3页Chinese Journal of Laboratory Diagnosis

基　　金：广东省卫生厅立项课题(A2011253)

摘　　要：目的探讨CD4+CD25nt/hi CD127lo调节性T细胞在哮喘儿童外周血中的比例改变,并探讨其临床意义。方法150例哮喘患儿按临床表现分为急性发作组(50例)、临床缓解期组(50例)和咳嗽变异性哮喘组(50例),另选择50名健康儿童为正常对照组。应用流式细胞仪检测上述各组外周血CD4+CD25nt/hi CD127lo调节性T细胞占CD4+T细胞的百分比。结果急性发作期组患儿外周血CD4+CD25nt/hi CD127lo调节性T细胞水平较缓解组、咳嗽变异性哮喘组及健康对照组明显下降(P<0.05),而缓解期组及咳嗽变异性哮喘组与健康对照组比较差异无统计学意义(P>0.05)。结论 CD4+CD25nt/hi CD127lo调节性T细胞可能参与了哮喘的发生与发展,哮喘的严重程度可能与CD4+CD25nt/hi CD127lo调节性T细胞的水平相关。Objective To explore the proportion change of CD4＋ CD25nt/hiCD127lo regulatory T eells（Treg）in pe- ripheral blood of children withasthma and to analyze its significance. Methods 150 asthmatic children were divided into three groups according to their clinicalfeatures：50 subjects in acute asthma attack group, 50 subjects in clinical remis- sion of asthma group and 50 subjects in cough variant asthma group, meanwhile, 50 healthy children were enrolled in the control group. The levels of CD4＋ CD25nt/hiCD127lo Treg in peripheral blood of all children were detected by flow cy- tometer. Results The CD4＋ CD25nt/hiCD127lo Treg level in acute attack group were lowest of the four groups （P〈 0.05） ,but the level in the control group was not significantly dif-ferent from the the clinical remission group and the cough variant asthma（all P〉0.05）. Conclusion The CD4＋ CD25nt/hiCD127lo regulatory T cells may be involved in the pathogenesis of asthma,The level of CD4＋ CD25nt/hiCD127lo regulatory T cells correlated with the severity of asthma in children.

关 键 词：哮喘 儿童 调节性T细胞 流式细胞术 

分 类 号：R725.6[医药卫生—儿科]